9 Result(s)
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Protocol
Mass Spectrometry-Based Methods to Determine the Substrate Specificities and Kinetics of N-Linked Glycan Hydrolysis by Endo-β-N-Acetylglucosaminidases
Glycosylation is a common posttranslational modification of proteins and refers to the covalent addition of glycans, chains of polysaccharides, onto proteins producing glycoproteins. The glycans influence the ...
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Protocol
Total Metabolome Extraction from Mycobacterial Cells for GC-MS Metabolomics Analysis
Over the past 10 years, the number of metabolomics based publications in the available scientific literature has exponentially grown, a large portion of which describing new biomarkers better elucidating micro...
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Protocol
Fatty Acid Metabolome Extraction from Mycobacterial Cells for GC-MS Metabolomics Analysis
Metabolomics is becoming an increasingly popular research tool for identifying new biomarkers, which can, among other applications, be applied to elucidate various microbial growth and virulence mechanisms. Si...
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Protocol
Co-immunoprecipitation Analysis for the Detection of Protein–Protein Interactions in Yersinia pestis
Co-immunoprecipitation is a classical method for the study of protein–protein and protein–DNA interactions, which is based on the specific recognition of antigens by antibodies (Sahr T, Buchrieser C: Methods M...
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Protocol
Identification of AI-2 Quorum Sensing Inhibitors in Vibrio harveyi Through Structure-Based Virtual Screening
Quorum sensing (QS) is a cell-to-cell communication system that regulates gene expression as a result of the production and perception of signal molecules called autoinducers (AIs). AI-2 is a QS autoinducer pr...
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Protocol
Analysis of Yops Secretion by Yersinia pestis Type III Secretion System
The type III secretion system (T3SS) is a highly conserved virulence mechanism that is widely distributed in Gram-negative bacteria. Three pathogenic Yersinia species share a common T3SS encoded by a 70-kb plasmi...
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Protocol
Detection of Type III Secretion System Needle Assembly in Yersinia pestis by Cross-Linking YscF
Type III secretion systems (T3SSs) are finely tuned molecular machines that are required for the virulence of many Gram-negative bacteria. Both the transcription and secretion of the Yersinia pestis Ysc T3SS are ...
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Protocol
piggyBac Transposon-Based Insertional Mutagenesis for the Fission Yeast Schizosaccharomyces pombe
Transposon-mediated insertional mutagenesis is a powerful tool for genetic screens. We have developed a piggyBac transposon-based mutagenesis system for the fission yeast Schizosaccharomyces pombe. Here, we descr...
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Protocol
Phagocytosis and Killing Assays for Candida Species
Both innate resistance and acquired cell-mediated immunity are involved in an anti-Candida response. Essential components of both the arms of the immune defense against infections by Candida spp. include phagocyt...
