6 Result(s)
within
Protocol
Li**g Du
Biomedicine
Immunology
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Protocol
A Nonviral piggyBac Transposon-Mediated Method to Generate Large-Scale CAR-NK Cells from Human Peripheral Blood Primary NK Cells
With the inherent antitumor function and unique “off-the-shelf” potential, genetically engineered human natural killer (NK) cells with chimeric antigen receptors (CARs) bear great promise for the treatment of ...
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Protocol
Purification of AcrAB-TolC Multidrug Efflux Pump for Cryo-EM Analysis
The cell envelope of Gram-negative bacteria comprises an outer membrane, a cytoplasmic inner membrane, and an interstitial space. The tripartite multidrug transporter AcrAB-TolC, which uses proton electrochemi...
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Protocol
Monitoring and Modulation of Inducible Foxp3+ Regulatory T-Cell Differentiation in the Lymph Nodes Draining the Small Intestine and Colon
The mucosa-draining lymphoid tissue favors differentiation of inducible Foxp3+ regulatory T cells. Adoptive transfer of T-cell receptor (TCR) transgenic (Tg) T cells is a powerful tool to study antigen-specific r...
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Protocol
Purification, Quantification, and Functional Analysis of Complement Factor H
Complement Factor H (FH) is an abundant, non-enzymic plasma/serum glycoprotein, which has a major role in regulating activation of the complement system. It can be purified from human plasma/serum by affinity ...
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Protocol
ENU Mutagenesis in Mice
Forward genetics has led to many “breakthrough” discoveries, and with the mouse genome almost fully sequenced, the creation of phenotypes through random germline mutagenesis has become an efficient means by wh...
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Protocol
Isolation of Human Skin Dendritic Cells by In Vitro Migration
In the human skin, various types of antigen-presenting cells (APC) are present. In the epidermis, they are identified ultrastructurally as Langerhans cells (LC) by the presence of Birbeck granules. LC are cons...
