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    Protocol

    Identification of Asp Isomerization in Proteins by 18O Labeling and Tandem Mass Spectrometry

    Isomerization of aspartic acid (Asp) to isoaspartic acid (isoAsp) via succinimide intermediate is a common route of degradation for proteins that can affect their structural integrity. As Asp/isoAsp is isobari...

    Jennifer Zhang, Viswanatham Katta in Therapeutic Proteins (2012)

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    Protocol

    Balloon Injury in Rats as a Model for Studying TRP Channel Contribution to Vascular Smooth Muscle Remodeling

    Many vascular occlusive diseases are characterized by endothelial dysfunction and phenotypic switch of vascular smooth muscle cell (VSMC) from quiescent contractile to proliferative migratory phenotypes. These...

    Wei Zhang, Mohamed Trebak in TRP Channels in Drug Discovery (2012)

  3. No Access

    Protocol

    TRPs to Cardiovascular Disease

    Transient receptor potential (TRP) is a large superfamily of cation channels comprising 28 members in mammals. TRP channels are ubiquitously expressed in human tissues, including the cardiovascular system wher...

    José C. González-Cobos, Xuexin Zhang, Rajender K. Motiani in TRP Channels in Drug Discovery (2012)