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Protocol
Identification of Asp Isomerization in Proteins by 18O Labeling and Tandem Mass Spectrometry
Isomerization of aspartic acid (Asp) to isoaspartic acid (isoAsp) via succinimide intermediate is a common route of degradation for proteins that can affect their structural integrity. As Asp/isoAsp is isobari...
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Protocol
Balloon Injury in Rats as a Model for Studying TRP Channel Contribution to Vascular Smooth Muscle Remodeling
Many vascular occlusive diseases are characterized by endothelial dysfunction and phenotypic switch of vascular smooth muscle cell (VSMC) from quiescent contractile to proliferative migratory phenotypes. These...
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Protocol
TRPs to Cardiovascular Disease
Transient receptor potential (TRP) is a large superfamily of cation channels comprising 28 members in mammals. TRP channels are ubiquitously expressed in human tissues, including the cardiovascular system wher...