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    Protocol

    SpyTag–SpyCatcher Chemistry for Protein Bioconjugation In Vitro and Protein Topology Engineering In Vivo

    The emergence of “molecular superglue,” such as SpyTag–SpyCatcher chemistry, has tremendously expanded our capability in manipulating protein shape and architecture via conjugation. Telechelic proteins bearing...

    **ao-Wei Wang, Wen-Bin Zhang in Bioconjugation (2019)

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    Protocol

    Site-Selective Enzymatic Labeling of Designed Ankyrin Repeat Proteins Using Protein Farnesyltransferase

    Affinity agents coupled to a functional moiety play an ever-increasing role in modern medicine, ranging from radiolabeled selective binders in diagnosis to antibody–drug conjugates in targeted therapies. In bi...

    Yi Zhang, Shelby Auger, Jonas V. Schaefer, Andreas Plückthun… in Bioconjugation (2019)