6 Result(s)
within
Protocol
Jian-** Guo
-
Protocol
Computational Analysis of Genome-Wide ARGONAUTE-Dependent DNA Methylation in Plants
Whole-genome bisulfite sequencing (WGBS) has become a powerful tool to dissect genome-wide methylation profiles at single-base resolution. In this chapter we describe in detail the bioinformatics pipeline used...
-
Protocol
Dissecting the Subnuclear Localization Patterns of Argonaute Proteins and Other Components of the RNA-Directed DNA Methylation Pathway in Plants
RNA-directed DNA methylation (RdDM) is a nuclear pathway which is comprised of multiple main and accessory protein components, including two plant-specific DNA-dependent RNA polymerases, Pol IV and Pol V, and ...
-
Protocol
Cloning and Profiling of Small RNAs from Cucumber Mosaic Virus Satellite RNA
RNA silencing is not only a gene regulation mechanism that is conserved in a broad range of eukaryotes but also an adaptive immune response against foreign nucleic acids including viruses in plants. A major fe...
-
Protocol
Micropatterning Cell Adhesion on Polyacrylamide Hydrogels
Cell shape and substrate rigidity play critical roles in regulating cell behaviors and fate. Controlling cell shape on elastic adhesive materials holds great promise for creating a physiologically relevant cul...
-
Protocol
Detection of Endogenous SOCS1 and SOCS3 Proteins by Immunoprecipitation and Western Blot Analysis
The suppressors of cytokine signalling (SOCS) protein family consist of eight members (SOCS 1–7, and CIS). SOCS1 and SOCS3 are the best-studied family members and have been shown to act as negative feedback in...
-
Protocol
Quantitative Trait Loci Map**
This chapter presents current methods for map** quantitative trait loci (QTLs) in natural populations especially in humans. We discussed the experimental designs for QTL map**, traditional methods adopted ...
