15 Result(s)
within
Protocol
Hong-**g Zhang
-
Protocol
Cell Biological Analyses of Anther Morphogenesis and Pollen Viability in Arabidopsis and Rice
Major advances have been made in our understanding of anther developmental processes in flowering plants through a combination of genetic studies, cell biological technologies, biochemical analyses, microarray...
-
Protocol
New Generation of Photosensitizers Based on Inorganic Nanomaterials
Advance of nanomaterials and nanotechnology has offered new possibilities for photodynamic therapy (PDT). Large amount of different kinds of sensitizers and targeting moieties can now be loaded in nanometer’s ...
-
Protocol
Highly Efficient Genome Editing in Clostridium difficile Using the CRISPR-Cpf1 System
Clostridium difficile is often the primary cause of nosocomial diarrhea, leading to thousands of deaths annually worldwide. The availability of an efficient genome editing tool for C. difficile is essential to un...
-
Protocol
Utilizing Potato Virus X to Monitor RNA Movement
Mobility assays coupled with RNA profiling have revealed the presence of hundreds of full-length non-cell-autonomous messenger RNAs that move through the whole plant via the phloem cell system. Monitoring the ...
-
Protocol
LabCaS for Ranking Potential Calpain Substrate Cleavage Sites from Amino Acid Sequence
Calpains are a family of Ca2+-dependent cysteine proteases involved in many important biological processes, where they selectively cleave relevant substrates at specific cleavage sites to regulate the function of...
-
Protocol
Investigating Transgene Integration and Organization in Cotton (Gossypium hirsutum L.) Genome
In this chapter, we present detailed experimental procedures for investigating integration patterns of transgenes in cotton genome. We use conventional PCR and genomic Southern blot hybridization to characteri...
-
Protocol
Gene Silencing of Human Sertoli Cells Utilizing Small Interfering RNAs
Sertoli cells, as the unique somatic cells within the seminiferous tubules, play essential roles in regulating normal spermatogenesis. In addition, recent studies have demonstrated that Sertoli cells could hav...
-
Protocol
Determination of the Substrate Specificity of Protein Kinases with Peptide Micro- and Macroarrays
Elucidation of the key determinants for the phosphorylation site specificities of protein kinases facilitates identification of their physiological substrates, and serves to better define their critical roles ...
-
Protocol
Monitoring Protein Kinase Expression and Phosphorylation in Cell Lysates with Antibody Microarrays
Fuelled by advances in our understanding of the human kinome and phosphoproteome and the increasing availability of pan- and phosphosite-specific antibodies, antibody microarrays have emerged as powerful tools...
-
Protocol
Cell Biological Analyses of Anther Morphogenesis and Pollen Viability in Arabidopsis and Rice
Major advances have been made in recent years in our understanding of anther development through a combination of genetic studies, cell biological technologies, biochemical analysis, microarray and high-throug...
-
Protocol
Serial Transplantation of Bone Marrow to Test Self-renewal Capacity of Hematopoietic Stem Cells In Vivo
Hematopoietic stem cells (HSCs) have the ability to self-renew and replenish the blood and immune system for the life span of an individual. An age-associated decline in HSC function is responsible for the dec...
-
Protocol
Investigating Transgene Integration and Organization in Cotton (Gossypium hirsutum L.) Genome
In this chapter, we present detailed experimental procedures for investigating integration patterns of transgenes in cotton genome. We use conventional PCR and genomic Southern blot hybridization to characteri...
-
Protocol
Integration of Protein Kinases into Transcription Complexes: Identifying Components of Immobilised In Vitro Pre-initiation Complexes
Regulation of gene expression is essential for coordinated cell growth and development. The de-regulation of certain genes is also recognised to contribute to both heritable and acquired disease. Transcription...
-
Protocol
Analysis of Microdissected Cells by Two-Dimensional LC-MS Approaches
Laser capture microdissection (LCM) is a powerful tool that enables the isolation of specific cell types from tissue sections, overcoming the problem of tissue heterogeneity and contamination. We combined the ...
-
Protocol
μParaflo™ Biochip for Nucleic Acid and Protein Analysis
We describe in this chapter the use of oligonucleotide or peptide microarrays (arrays) based on microfluidic chips. Specifically, three major applications are presented: (1) microRNA/small RNA detection using ...
