5 Result(s)
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Protocol
Gene Silencing of Human Sertoli Cells Utilizing Small Interfering RNAs
Sertoli cells, as the unique somatic cells within the seminiferous tubules, play essential roles in regulating normal spermatogenesis. In addition, recent studies have demonstrated that Sertoli cells could hav...
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Protocol
Serial Transplantation of Bone Marrow to Test Self-renewal Capacity of Hematopoietic Stem Cells In Vivo
Hematopoietic stem cells (HSCs) have the ability to self-renew and replenish the blood and immune system for the life span of an individual. An age-associated decline in HSC function is responsible for the dec...
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Protocol
Integration of Protein Kinases into Transcription Complexes: Identifying Components of Immobilised In Vitro Pre-initiation Complexes
Regulation of gene expression is essential for coordinated cell growth and development. The de-regulation of certain genes is also recognised to contribute to both heritable and acquired disease. Transcription...
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Protocol
Analysis of Microdissected Cells by Two-Dimensional LC-MS Approaches
Laser capture microdissection (LCM) is a powerful tool that enables the isolation of specific cell types from tissue sections, overcoming the problem of tissue heterogeneity and contamination. We combined the ...
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Protocol
μParaflo™ Biochip for Nucleic Acid and Protein Analysis
We describe in this chapter the use of oligonucleotide or peptide microarrays (arrays) based on microfluidic chips. Specifically, three major applications are presented: (1) microRNA/small RNA detection using ...
