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Protocol
SWATH-MS Proteomic Approach to Discover Novel Protein Targets and Pathways in Response to Abscisic Acid
SWATH-MS proteomic approaches enable the identification and quantification of thousands of proteins within a single profiling experiment, which is useful for the identification of genes regulated by abscisic a...
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Protocol
Examination of Galectins in Phagocytosis
Galectins, a family of β-galactoside-binding proteins, are expressed in many different phagocytic leukocytes (granulocytes, monocytes, and macrophages). A number of family members have been shown to play an im...
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Protocol
Methods for Rice Phenomics Studies
With the completion of the rice genome sequencing project, the next major challenge is the large-scale determination of gene function. A systematic phenotypic profiling of mutant collections will provide major...
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Protocol
Characterization of Oligosaccharides from Starch, Dextran, Cellulose, and Glycoproteins by Capillary Electrophoresis
Neutral oligosaccharides are complex in terms of their linkage, either in the linear or branched form. An enormous number of variants are therefore possible. A homopolymer of glucose with a glycosidic bond may...
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Protocol
Development of Female Contraceptive Vaccine Through DNA Inoculation of Human Chorionic Gonadotropin Beta Subunit (hCGß)
Human chorionic gonadotropin (hCG) has been considered as a primary target molecule for a contraceptive vaccine by the World Health Organization because of its physiological and temporal specificity. hCG is an...
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Protocol
Methods for the Studies of Drug Dissociation from DNA
In addition to binding affinity, the on and off rates of drug-DNA interactions are important in determining the biological activities of a drug For example, the rate of dissociation of a drug from DNA has been...