6 Result(s)
within
Protocol
Dong-**ao Yang
English
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Protocol
CRISPR/LbCas12a-Mediated Genome Editing in Soybean
Currently methods for generating soybean edited lines are time-consuming, inefficient, and limited to certain genotypes. Here we describe a fast and highly efficient genome editing method based on CRISPR-Cas12...
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Protocol
Application of Immunohistochemistry in Basic and Clinical Studies
Immunohistochemistry (IHC), also known as immunohistochemical staining, is an immune morphological analysis. It is a process of selectively identifying antigens (proteins) by antibodies in cells or tissue sect...
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Protocol
Genome-Wide Annotation of circRNAs and Their Alternative Back-Splicing/Splicing with CIRCexplorer Pipeline
Circular RNAs (circRNAs) derived from back-spliced exons were sporadically identified about 25 years ago, and have been recently re-discovered genome-wide across different species. Interestingly, one gene locu...
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Protocol
Inoculation and Scoring Methods for Rice Sheath Blight Disease
Sheath blight disease of rice caused by the soilborne fungal pathogen Rhizoctonia solani has been a major disease of rice with a serious threat to stable rice production worldwide. Although various cultural pract...
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Protocol
Quantitative Trait Loci Map**
This chapter presents current methods for map** quantitative trait loci (QTLs) in natural populations especially in humans. We discussed the experimental designs for QTL map**, traditional methods adopted ...
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Protocol
cDNA Microarray and Bioinformatic Analysis of Nuclear Factor-κB Related Genes in Squamous Cell Carcinoma
Squamous cell carcinomas and several other cancers have been found to exhibit microarray expression profiles that include genes related to nuclear factor (NF)-κB, a signal activated transcription factor that i...
