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Correction to: Magnetic Resonance Spectroscopy (MRS) in Alzheimer’s Disease
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Machine Learning for Cerebrovascular Disorders
Cerebrovascular disease refers to a group of conditions that affect blood flow and the blood vessels in the brain. It is one of the leading causes of mortality and disability worldwide, imposing a significant ...
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Image Registration: Fundamentals and Recent Advances Based on Deep Learning
Registration is the process of establishing spatial correspondences between images. It allows for the alignment and transfer of key information across subjects and atlases. Registration is thus a central techn...
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A Regulatory Science Perspective on Performance Assessment of Machine Learning Algorithms in Imaging
This chapter presents a regulatory science perspective on the assessment of machine learning algorithms in diagnostic imaging applications. Most of the topics are generally applicable to many medical imaging a...
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Studying Annelida Regeneration in a Novel Model Organism: The Freshwater Aeolosoma viride
Aeolosoma viride, a globally distributed freshwater annelid, has a semitransparent appearance with 10 to 12 segments, about 2 to 3 mm in length. It is easy to raise and handle in laboratory conditions. Due to its...
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Monitoring Telomere Maintenance During Regeneration of Annelids
Telomere shortening is a hallmark of aging and eventually constrains the proliferative capacity of cells. The protocols discussed here are used for monitoring telomeres comprehensively in Aeolosoma viride, a mode...
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Manipulation of Gene Activity in the Regenerative Model Sea Anemone, Nematostella vectensis
With a surprisingly complex genome and an ever-expanding genetic toolkit, the sea anemone has become a powerful model system for the study of both development and whole-body . Here we provide the most curr...
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Bulk Sequencing from mRNA with UMI for Evaluation of B-Cell Isotype and Clonal Evolution: A Method by the AIRR Community
During the course of an immune response to a virus such as influenza, B cells undergo activation, clonal expansion, isotype switching, and somatic hypermutation (SHM). Members of an antigen-experienced B-cell ...
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Bulk gDNA Sequencing of Antibody Heavy-Chain Gene Rearrangements for Detection and Analysis of B-Cell Clone Distribution: A Method by the AIRR Community
In this method we illustrate how to amplify, sequence, and analyze antibody/immunoglobulin (IG) heavy-chain gene rearrangements from genomic DNA that is derived from bulk populations of cells by next-generatio...
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Correction to: Methods to Detect Immunogenic Cell Death In Vivo
This book was inadvertently published with one of the contributing author’s name printed as Aitziber Buqué Martinez, which should have been Aitziber Buqué.
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Study of MAIT Cell Activation in Viral Infections In Vivo
MAIT cells are abundant, highly evolutionarily conserved innate-like lymphocytes expressing a semi-invariant T cell receptor (TCR), which recognizes microbially derived small intermediate molecules from the ri...
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Erratum to: Microgrooved Surface Modulates Neuron Differentiation in Human Embryonic Stem Cells
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Erratum to: Enzyme-Free Dissociation of Neurospheres by a Microfluidic Chip-Based Method
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Erratum to: Functional Analysis of Histone Deacetylase 11 (HDAC11)
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PCR Amplification and Sequencing Analysis of Full-Length Turkey Coronavirus Spike Gene
Turkey coronaviral enteritis caused by turkey coronavirus (TCoV) continues to infect turkey flocks, resulting in significant economic loss. Determining and understanding genetic relationships among different T...
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Virus Neutralization Assay for Turkey Coronavirus Infection
Turkey coronavirus (TCoV) infection induces the production of protective antibodies against the sequent exposure of TCoV. Serological tests to determine TCoV-specific antibodies are critical to evaluate previo...
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Antibody-Capture Enzyme-Linked Immunosorbent Assay for Detection of Antibody to Turkey Coronavirus Using Infectious Bronchitis Virus or Recombinant Nucleocapsid Protein as Coating Antigen
Turkey coronavirus (TCoV) infection continues to threaten turkey industry. Because specific treatment and effective vaccination program are not available, rapid and cost-effective detection of antibodies to TC...
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Real-Time Reverse Transcription-Polymerase Chain Reaction for Detection and Quantitation of Turkey Coronavirus RNA in Feces and Intestine Tissues
Turkey coronavirus (TCoV) infection causes acute atrophic enteritis in turkey poults, leading to significant economic loss in the turkey industry. Rapid detection, differentiation, and quantitation of TCoV are...
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ERRATUM TO: Induction of Brain Arteriovenous Malformation in the Adult Mouse
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Identification of Viral Peptide Fragments for Vaccine Development
We report a simple method for identifying foldable viral surface protein fragments in a random but systematic manner. The method involves digestion and reassembly of a target gene to generate a pool of smaller...
