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  1. No Access

    Protocol

    Designing Overlap Extension PCR Primers for Protein Mutagenesis: A Programmatic Approach

    Overlap extension PCR is one of the routinely used methods to generate mutagenic genes for the functional and structural study of proteins. However, it is time-consuming to design the overlap** mutagenic pri...

    **aofang Huang, Liangting Xu, Chuyun Bi, Lili Zhao, Limei Zhang… in Directed Evolution (2022)

  2. No Access

    Protocol

    Site-Directed Mutagenesis Method Mediated by Cas9

    This study presents an in vitro CRISPR/Cas9-mediated mutagenic (ICM) system that allows rapid construction of designed mutants or site-saturation mutagenesis libraries in a PCR-independent manner. The plasmid ...

    Wan** Chen, Wenwen She, Aitao Li, Chao Zhai, Lixin Ma in Directed Evolution (2022)

  3. No Access

    Protocol

    Generation of Rabbit Models by Gene Editing Nucleases

    Due to the lack of germline transmitting pluripotent stem cells (PSCs) cell lines and the extreme difficulty of somatic cell nuclear transfer (SCNT) in rabbit, the gene targeting technology in rabbit was laggi...

    Dongshan Yang, Jie Xu, Y. Eugene Chen in Microinjection (2019)

  4. No Access

    Protocol

    An Emulsion Polymerase Chain Reaction–Based Method for Molecular Haploty**

    Genotypes are easily measured using a variety of experimental methods. However, experimental methods for measuring haplotypes, i.e., molecular haploty**, are limited. Instead, haplotypes often are statistica...

    James G. Wetmur, Jia Chen in Environmental Genomics (2008)

  5. No Access

    Protocol

    Forward-Phase and Reverse-Phase Protein Microarray

    Protein microarray is a powerful tool for identifying disease biomarkers and therapeutical targets, and for systematically studying biological pathways with high efficiency. Although the protein microarray pla...

    Ya** Zong, Shanshan Zhang, Huang-Tsu Chen, Yunfei Zong, Yaxian Shi in Microarrays (2007)

  6. No Access

    Protocol

    Microchip Electrophoresis for DNA Separation by Wire-Imprinted Microchannels on PMMA Substrates

    Microchip electrophoresis has become a mature separation technique in recent years. Compared to agarose gel electrophoresis, which is commonly used for DNA separation, microchip electrophoresis has several adv...

    Shu-Hui Chen in Microchip-Based Assay Systems (2007)

  7. No Access

    Protocol

    Nanotechnology

    Microarrays are important tools for high-throughput analysis of biomolecules. The use of microarrays for parallel screening of nucleic acid and protein profiles has become an industry standard. A few limitatio...

    Hua Chen, Jun Li in Microarrays (2007)