6 Result(s)
within
English
Biological Techniques
Biotechnology
Da** Chen
Protocol
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Protocol
Structure-Guided and Phage-Assisted Evolution of Therapeutic Antibodies to Reverse On-Target Point Mutation-Mediated Resistance
Resistance to therapeutic antibodies caused by on-target point mutations is a major obstacle in anticancer therapy, creating an “unmet clinical need.” To tackle this problem, researchers are develo** new gen...
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Protocol
Preparation of Bioconjugates of Chimeric M13 Phage and Gold Nanorods
Phage-nanomaterial conjugates are functional bio-nanofibers with various applications. While phage display can select for phages with desired genetically encoded functions and properties, nanomaterials can end...
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Protocol
Enzymatic Preparation of DNA with an Expanded Genetic Alphabet Using Terminal Deoxynucleotidyl Transferase and Its Applications
Efficient preparation of DNA oligonucleotides containing unnatural nucleobases (UBs) that can pair with their cognates to form unnatural base pairs (UBPs) is an essential prerequisite for the application of UB...
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Protocol
Visualization of Engineered M13 Phages Bound to Bacterial Targets by Transmission Electron Microscopy
The filamentous phage M13 is one of the most well-studied and characterized phages, particularly since it was introduced as a scaffold for phage display, a technique to express and evolve fusion proteins on th...
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Protocol
Construction of Xylose-Utilizing Cyanobacterial Chassis for Bioproduction Under Photomixotrophic Conditions
Xylose is a major component of lignocellulose and the second most abundant sugar present in nature after glucose; it, therefore, has been considered to be a promising renewable resource for the production of b...
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Protocol
Vaccine Delivery with a Detoxified Bacterial Toxin
It is still a challenge to develop needle-free mucosal vaccines. Despite progress in the development of the influenza vaccine, it must be reformulated annually because of antigenic changes in circulating influ...
