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    Cellular Approaches in Investigating Argonaute2-Dependent RNA Silencing

    In mammals, there are four Argonaute (Ago) family proteins that play crucial roles in RNA silencing, a process wherein microRNA (miRNA) mediates inhibition of target mRNA translation. Among the Ago proteins, A...

    Cai Zhang, Joonbae Seo, Takahisa Nakamura in Argonaute Proteins (2018)

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    Protocol

    Conversion of Non-endocrine Human Pancreatic Cells to Insulin-Producing Cells for Treatment of Diabetes

    Type I diabetes results from the autoimmune destruction of the insulin-secreting pancreatic β-cells, affecting many millions of people worldwide. The optimal treatment is to restore the endogenous supply of in...

    Min Zhao, Guo Cai Huang in Human Cell Culture Protocols (2012)

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    Protocol

    Proteolysis of Cortactin by Calpain in Platelets and In Vitro

    Circulating platelets undergo dramatic morphological changes in response to platelet agonists, resulting in attachment, spreading, secretion, and aggregation. These rapid dynamic physiological activities requi...

    Cai Huang, ** Zhan in Calpain Methods and Protocols (2000)