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    Protocol

    Imaging of Human Cancer Cell Proliferation, Invasion, and Micrometastasis in a Zebrafish Xenogeneic Engraftment Model

    The xenograft model, using the early life stages of the zebrafish, allows imaging of tumor cell behavior both on a single cell and whole organism level, over time, within a week. This robust and reproducible a...

    Claudia Tulotta, Shuning He, Lanpeng Chen, Arwin Groenewoud in Zebrafish (2016)

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    Protocol

    RNA Sequencing of FACS-Sorted Immune Cell Populations from Zebrafish Infection Models to Identify Cell Specific Responses to Intracellular Pathogens

    The zebrafish (Danio rerio) is increasingly used as a model for studying infectious diseases. This nonmammalian vertebrate host, which is transparent at the early life stages, is especially attractive for live im...

    Julien Rougeot, Ania Zakrzewska, Zakia Kanwal, Hans J. Jansen in Host-Bacteria Interactions (2014)

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    Protocol

    Yeast One-Hybrid Screens for Detection of Transcription Factor DNA Interactions

    The yeast one-hybrid system is widely recognized as a valuable and straightforward technique to study interactions between transcription factors and DNA. By means of one-hybrid screens, transcription factors o...

    Pieter B. F. Ouwerkerk, Annemarie H. Meijer in Plant Reverse Genetics (2011)