1,162 Result(s)
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Protocol
Nanoparticle Size Distribution and Stability Assessment Using Asymmetric-Flow Field-Flow Fractionation
Nanomaterials are inherently polydisperse. Traditional techniques, such as the widely used batch-mode dynamic light-scattering (DLS) analysis, are not ideal nor thoroughly descriptive enough to define the full...
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Protocol
Detection of Induction of Mitochondrial Oxidative Stress by Nanoparticles in T Cells Using MitoSOX Red Dye
The induction of oxidative stress by engineered nanomaterials has been associated with cytotoxic and inflammatory responses, damaging healthy cells and tissues. In contrast, when directed against cancer and au...
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Protocol
Deep Learning-Based HLA Allele Imputation Applicable to GWAS
Human leukocyte antigen (HLA) imputation is an essential step following genome-wide association study, particularly when putative associations in HLA genes are identified, to fully understand the genetic basis...
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Protocol
Generation of Orthotopic and Subcutaneous Patient-Derived Xenograft Models from Diverse Clinical Tissue Samples of Pediatric Extracranial Solid Tumors
Realistic and renewable laboratory models that accurately reflect the distinct clinical features of childhood cancers have enormous potential to speed research progress. These models help us to understand dise...
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Protocol
Echocardiography to Assess Cardiac Structure and Function in Genetic Cardiomyopathies
Rodents are the most common experimental models used in cardiovascular research including studies of genetic cardiomyopathies. Genetic cardiomyopathies are characterized by changes in cardiac structure and fun...
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Protocol
Knowledge Graphs and Their Applications in Drug Discovery
Knowledge graphs represent information in the form of entities and relationships between those entities. Such a representation has multiple potential applications in drug discovery, including democratizing acc...
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Protocol
A Multiplexed Approach to Assess Small Cell Lung Cancer Subtype Heterogeneity in Primary and Patient-Derived Tumor Samples
Unlocking the heterogeneity of cancers is crucial for develo** therapeutic approaches that effectively eradicate disease. As our understanding of markers specific to cancer subclones or subtypes expands, the...
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Protocol
Quantitation of Active Pharmaceutical Ingredients in Polymeric Drug Products Using Elemental Analysis
Polymeric prodrugs have gained significant popularity as a strategy to enhance the bioavailability and improve the pharmacokinetic properties of active pharmaceutical ingredients (API). Since the amount of the...
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Protocol
Detection of Antigen Presentation by Murine Bone Marrow–Derived Dendritic Cells After Treatment with Nanoparticles
Nanoparticles are frequently considered in vaccine applications due to their ability to co-deliver multiple antigens and adjuvants to antigen-presenting cells. Some nanoparticles also have intrinsic adjuvant p...
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Protocol
Analysis of Nanoparticle Adjuvant Properties
Nanoparticles can be engineered for targeted antigen delivery to immune cells and for stimulating an immune response to improve the antigen immunogenicity. This approach is commonly used to develop nanotechnol...
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Protocol
HLA Ty** and Mutation Calling from Normal and Tumor Whole Genome Sequencing Data with ALPHLARD-NT
HLA somatic mutations can alter the expression and function of HLA molecules, which in turn affect the ability of the immune system to recognize and respond to cancer cells. Therefore, it is crucial to accurat...
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Protocol
Natural Language Processing for Drug Discovery Knowledge Graphs: Promises and Pitfalls
Building and analyzing knowledge graphs (KGs) to aid drug discovery is a topical area of research. A salient feature of KGs is their ability to combine many heterogeneous data sources in a format that facilita...
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Protocol
A Humanized Patient-Derived Xenograft Model for Pancreatic Cancer
Pancreatic cancer is associated with a high mortality rate, and there are still very few effective treatment options. Patient-derived xenografts have proven to be invaluable preclinical disease models to study...
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Protocol
Atomistic Simulations of Sarcomere Proteins
Concerted atomic motions are requisite for sarcomere protein function and may become disrupted in HCM pathologies. Computational approaches such as molecular dynamics simulation can resolve such dynamics with ...
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Protocol
Patient-Derived Xenograft Models for Translational Prostate Cancer Research and Drug Development
Patient-derived xenografts (PDXs) are a valuable preclinical research platform generated through transplantation of a patient’s resected tumor into an immunodeficient or humanized mouse. PDXs serve as a high-f...
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Protocol
Isolation and Immunophenoty** of Leukocytes from the Human Maternal-Fetal Interface
During human pregnancy, leukocytes that infiltrate the maternal-fetal interface play a major role in establishing a delicate balance between immune tolerance and functional response and setting the inflammator...
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Protocol
Amyloid PET Imaging: Standard Procedures and Semiquantification
Amyloid plaques are a neuropathologic hallmark of Alzheimer’s disease (AD), which can be imaged through positron emission tomography (PET) technology using radiopharmaceuticals that selectively bind to the fib...
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Protocol
Measuring Size and Number Concentration of Metallic Nanoparticles Using spICP-MS
This protocol describes how to measure the size and concentration of individual metallic nanoparticles using inductively coupled plasma-mass spectrometry (ICP-MS) in single-particle (sp) mode. Accurately deter...
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Protocol
Detection of Pre-Existing Antibodies to Polyethylene Glycol and PEGylated Liposomes in Human Serum
Polyethylene glycol, or PEG, is common in consumer products, over-the-counter medications, food, and pharmaceutical products. Concerns about PEG immunogenicity and the subsequent negative impact of pre-existin...
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Protocol
Evaluation of the Acute Anaphylactoid Reactogenicity of Nanoparticle-Containing Medicines and Vaccines Using the Porcine CARPA Model
A small fraction, up to 10%, of people treated intravenously with state-of-the-art nanoparticulate drugs or diagnostic agents develop an acute infusion reaction which can be severe or even lethal. Activation o...
