17 Result(s)
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English
Chemistry
Biochemistry, general
Bioinformatics
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Protocol
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Protocol
Synthetic Strategies for FRET-Enabled Carbohydrate Active Enzyme Probes
Carbohydrates are an essential class of biomolecule and carbohydrate active enzymes (CAZys) catalyze their synthesis, refinement, and degradation, hence contributing an overall regulatory capacity to their und...
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Lectin Histochemistry for Tissues and Cells, and Dual Lectin and Antibody Co-localization
In this chapter we describe in detail methods for lectin staining of (1) tissues, and (2) cells to identify and map endogenous glycosylation. We also describe (3) dual antibody and lectin staining of tissues t...
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Metabolic Labeling of Primary Neurons Using Carbohydrate Click Chemistry
Glycans play an important role in many neuronal processes, such as neurotransmitter release and reuptake, cell–cell communication and adhesion, modulation of ion channel activity, and immune function. Carbohyd...
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Glycoinformatics Tools for Comprehensive Characterization of Glycans Enzymatically Released from Proteins
Glycosylation is important in biology, contributing to both protein conformation and function. Structurally, glycosylation is complex and diverse. This complexity is reflected in the topology, composition, mon...
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An Interactive View of Glycosylation
The present chapter focuses on the interactive and explorative aspects of bioinformatics resources that have been recently released in glycobiology. The comparative analysis of data in a field where knowledge ...
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Comparative Glycomics Analysis of Mass Spectrometry Data
Glycan profiling is a common strategy that is used to determine the distribution of N-linked glycans, O-linked glycans and glycolipid associated complex carbohydrate structures that are part of various cell an...
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Rapid Antibody Glycoengineering in CHO Cells Via RNA Interference and CGE-LIF N-Glycomics
The impact of the glycan distribution on the in vivo function and half-life of has long motivated the genetic engineering of producer cells to achieve structures that enhance efficacy, safety and stability. ...
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Recent Advances Toward Engineering Glycoproteins Using Modified Yeast Display Platforms
Yeast are capable recombinant protein expression hosts that provide eukaryotic posttranslational modifications such as disulfide bond formation and N-glycosylation. This property has been used to create surface d...
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O-Glycologue: A Formal-Language-Based Generator of O-Glycosylation Networks
The web application O-Glycologue provides an online simulation of the biosynthetic enzymes of O-linked glycosylation, using a knowledge-based system described previously. Glycans can be imported in GlycoCT conden...
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N-glycan Characterization by Liquid Chromatography Coupled with Fluorimetry and Mass Spectrometry
Human blood plasma and serum have been a source of biomarkers for the indication and progression of many diseases for a few decades now. Human blood plasma is also an excellent source material to enable patien...
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Lectin Affinity Chromatography for the Discovery of Novel Cancer Glycobiomarkers: A Case Study with PSA Glycoforms and Prostate Cancer
Many clinical biomarkers in cancer are glycoproteins, but the majority of them only consider the protein levels. Indeed, only alfa-fetoprotein (AFP) in hepatocarcinoma and CA15-3 in breast cancer are clinicall...
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Monitoring the Sialome on Human Immune Cells
The sialome or display of sialic acids on the surface of human immune cells can vary according to immune response and activation state. Here, human peripheral blood mononuclear cells (PBMCs) were isolated and ...
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GlycoStore: A Platform for H/UPLC and Capillary Electrophoresis Glycan Data
GlycoStore (http://www.glycostore.org) is an open access chromatographic and electrophoretic retention database of glycans characterized from glycoproteins, glycol...
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Characterization and Analysis of Food-Sourced Carbohydrates
Food carbohydrates are macronutrients that are found in fruits, grains, vegetables, and milk products. These organic compounds are present in foods in the form of sugars, starches, and fibers and are composed ...
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Cell Free Remodeling of Glycosylation of Antibodies
The N-glycosylation profile of a monoclonal antibody (mAb) is a critical quality attribute in relation to its therapeutic application. The control of this profile during biomanufacture is difficult because of the...
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In Silico Analysis of Therapeutic Antibody Aggregation and the Influence of Glycosylation
The aggregation of therapeutic antibodies is a major issue for the pharmaceutical industry leading to loss of drug quality, increased dosage, and unwanted immune responses such as the production of anti-drug a...
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Computational Modeling of Glycan Processing in the Golgi for Investigating Changes in the Arrangements of Biosynthetic Enzymes
Modeling glycan biosynthesis is becoming increasingly important due to the far-reaching implications that glycosylation can exhibit, from pathologies to biopharmaceutical manufacturing. Here we describe a ap...
