16 Result(s)
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Biomedicine
Bioinformatics
Cell Biology
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Protocol
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Protocol
Handling High-Throughput Omics Data for Systems Genetics Analysis
Omics data are being generated and collected at unprecedented scale. During the last decade, single omics, such as genomics, transcriptomics, proteomics, and metabolomics, have already highlighted pathophysiol...
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Cytotoxic T Lymphocytes (CTLs) and Kidney Transplantation: An Overview
Involvement of T lymphocytes in kidney transplantation is a well-developed topic; however, most of the scientific interest focused on the different type of CD4+ lymphocyte subpopulations. Few authors, instead,...
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Gut Microbiota, the Immune System, and Cytotoxic T Lymphocytes
Gut microbiota, the largest microbial community living in the human body, exerts a variety of metabolic, structural, and functional actions. In particular, it is essential for the full immune system developmen...
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Phenotypical and Functional Characterization of Cytotoxic Unconventional T-Cells
During the last two decades, the immunology field has been focused on the study of conventional T-cells, leading to important advances in identification of specific subsets involved in promoting and suppressin...
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Antigen-Specific In Vivo Killing Assay
The in vivo killing assay allows the quantification of the antigen-specific killing capacity of Cytotoxic CD8+ T Lymphocytes (CTLs) in mice. CTLs are indeed known for the lysis of cells expressing foreign or modi...
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Methods for Characterization of Senescent Circulating and Tumor-Infiltrating T-Cells: An Overview from Multicolor Flow Cytometry to Single-Cell RNA Sequencing
Immunosenescence is the general term used to describe the aging-associated decline of immunological function that explains the higher susceptibility to infectious diseases and cancer, increased autoimmunity, o...
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Characterization of Cytotoxic T Lymphocytes (CTL) by Tissue Microarray
Tissue microarray (TMA) is a smart technical innovation recently imposed in pathology research. This technology provides a high-throughput analysis of multiple tissues at the same time. The technique allows fa...
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Whole Exome Sequencing for the Identification of Mutations in CD8+ T-Cells
Advances in next-generation sequencing and in particular whole exome sequencing (WES) have provided an innovative opportunity to perform a mutational screening of the entire coding region of the genome down to...
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Analysis of miRNA Expression Using Digital PCR and the QuantStudio™ 3D Digital PCR System
miRNAs are short, single-stranded RNA molecules that function as posttranscriptional regulators of gene expression. miRNAs represent ideal biomarkers since they can also circulate in the bloodstream as well as...
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Protein-Bound Uremic Toxins and Immunity
Protein-bound uremic toxins (PBUTs) are bioactive microbiota metabolites originated exclusively from protein fermentation of the bacterial community resident within the gut microbiota, whose composition and fu...
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Flow Cytometry Instrument Setting as a Crucial Checkpoint for Optimal T-Cell Analysis and Sorting
Although flow cytometry and cell sorting are widely used by immunologists both for basic and translation research, many aspects of these techniques should be optimized to obtain reproducible and meaningful dat...
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Tumor Infiltrating T Cell Cytotoxicity Assay
Cytotoxicity is the primary function of CD8+ T-cells, also called cytotoxic CD8+ T lymphocytes (or CTLs). Quantification of this capacity is of major importance in diagnostic and research tools. While phenotypic ...
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CTL ELISPOT Assay and T Cell Detection
Enzyme-linked immune absorbent spot (Elispot) is a quantitative method for measuring relevant parameters of T-cell activation. The sensitivity of Elispot allows the detection of low-frequency antigen-specific ...
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In Vivo and In Vitro Models for the Study of Bone Remodeling and the Role of Immune Cells
Immune and bone cells cross talk has been established by different years; however the underlying mechanisms require continuous investigation. To this end both in vivo and in vitro models have been realized and...
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A Review of Proteomics Strategies to Study T-Cell Activation and Function in Cancer Disease
Cytotoxic T-cells play a key role in natural response to cancer and in immunotherapy. Understanding in an ever more thorough and complete way the mechanisms underlying their activation and/or those that preven...
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Methods to Study Posttranslational Modification Patterns in Cytotoxic T-Cells and Cancer
Protein posttranslational modifications (PTMs) regulate intracellular signaling associated with development and progression of many diseases; thus, they are key to understanding pathological mechanisms and set...
