473 Result(s)
within
English
Life Sciences
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Correction to: ROS Signaling in Plants
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Corrections to: Plastids
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Correction to: Tissue-Resident Macrophages: Methods and Protocols
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Correction to: Xylem: Methods and Protocols
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Ready-to-Record Cells for Kinetic Screening of VGICs
Voltage-gated ion channels (VGICs) are integral membrane proteins crucial for transmitting electrical signals in excitable cells. Understanding the kinetics of these ion channels requires conducting patch-clam...
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Use of Surface Plasmon Resonance Technique for Studies of Inter-domain Interactions in Ion Channels
Ion channels are transmembrane proteins essential for cellular functions and are important drug targets. Surface plasmon resonance (SPR) is a powerful technique for investigating protein–protein and protein–sm...
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A Practical Approach to Using the Genomic Standards Consortium MIxS Reporting Standard for Comparative Genomics and Metagenomics
Comparative analysis of (meta)genomes necessitates aggregation, integration, and synthesis of well-annotated data using standards. The Genomic Standards Consortium (GSC) collaborates with the research communit...
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Correction to: Exosomal MicroRNAs: Comprehensive Methods from Exosome Isolation to miRNA Extraction and Purity Analysis
In the original version of this book, the first and the last names of the authors of Chapter 5 were in a flipped format (D’Agostino Erika, Muro Annamaria, Sgueglia Giulia, Massaro Crescenzo, Dell’Aversana Carm...
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Correction to: Advanced Technologies and Automation in mES Cell Workflow
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Correction to: Golgi
The original version of the book was inadvertently published without incorporating the author’s proof corrections mentioned below. The chapters have now been corrected and approved by the author.
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Correction to: Cloning by SCNT: Integrating Technical and Biology-Driven Advances
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Measuring CTL Lytic Granule Secretion and Target Cell Membrane Repair by Fluorescent Lipophilic Dye Uptake at the Lytic Synapse
CD8+ cytotoxic T lymphocytes (CTL) play a key role in anti-tumor immune response. They are therefore at the heart of current immunotherapy protocols against cancer. Despite current strategies to potentiate CTL re...
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Methods of Machine Learning-Based Chimeric Antigen Receptor Immunological Synapse Quality Quantification
Chimeric Antigen Receptor (CAR)-mediated immunotherapy shows promising results for refractory blood cancers. Currently, six CAR-T drugs have been approved by U.S. Food and Drug Administration (FDA). Theoretica...
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Separation of Single Core and Multicore Lytic Granules by Subcellular Fractionation and Immunoisolation
Subcellular fractionation is an important tool used to separate intracellular organelles, structures or proteins. Here, we describe a stepwise protocol to isolate two types of lytic granules, multicore (MCG), ...
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Profiling Chromatin Accessibility on Replicated DNA with repli-ATAC-Seq
Open or accessible chromatin typifies euchromatic regions and helps define cell type-specific transcription programs. DNA replication massively disorders chromatin composition and structure, and how accessible...
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Read-Based Phasing and Analysis of Phased Variants with WhatsHap
WhatsHap is a command-line tool for phasing and phasing-related tasks. It allows to infer haplotypes in diploid and polyploid samples based on (preferably long) reads covering at least two heterozygous variant...
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Exploiting the RUSH System to Study Lytic Granule Biogenesis in Cytotoxic T Lymphocytes
The Retention Using Selective Hooks (RUSH) system allows for the synchronized release of one or more proteins of interest from a donor endomembrane compartment, usually the endoplasmic reticulum, and the subseque...
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High-Throughput Production and Optimization of Membrane Proteins After Expression in Mammalian Cells
High-quality protein samples are an essential requirement of any structural biology experiment. However, producing high-quality protein samples, especially for membrane proteins, is iterative and time-consumin...
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The Origin of Left-Handed Poly[d(G-C)]
The discovery of a reversible transition in the helical sense of a double-helical DNA was initiated by the first synthesis in 1967 of the alternating sequence poly[d(G-C)]. In 1968, exposure to high salt conce...
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Visualizing and Quantifying mRNA Localization at the Invasive Front of 3D Cancer Spheroids
Localization of mRNAs at the front of migrating cells is a widely used mechanism that functionally supports efficient cell movement. It is observed in single cells on two-dimensional surfaces, as well as in mu...
