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    Convenient and Efficient Enrichment of the CD133+ Liver Cells from Rat Fetal Liver as a Source of Liver Stem/Progenitor Cells

    Although stem cells are commonly isolated by fluorescence-activated cell sorting or magnetic affinity cell sorting, they are very expensive, and they need known markers. However, there is no specific marker fo...

    Weihui Liu, Nan You, Kefeng Dou in Somatic Stem Cells (2012)

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    Protocol

    Prediction of Protein Phosphorylation Sites by Integrating Secondary Structure Information and Other One-Dimensional Structural Properties

    Studies on phosphorylation are important but challenging for both wet-bench experiments and computational studies, and accurate non-kinase-specific prediction tools are highly desirable for whole-genome annota...

    Yongchao Dou, Bo Yao, Chi Zhang in Prediction of Protein Secondary Structure (2017)

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    Protocol

    Experimentally Validated Plant lncRNAs in EVLncRNAs Database

    Plant long noncoding RNAs (lncRNAs) play important functional roles in various biological processes. Most databases deposit all plant lncRNA candidates produced by high-throughput experimental and/or computati...

    Bailing Zhou, Huiying Zhao, Jiafeng Yu, Chengang Guo in Plant Long Non-Coding RNAs (2019)

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    Protocol

    Pictet–Spengler Reaction for the Chemical Synthesis of Strictosidine

    Strictosidine is the common biosynthetic precursor of Monoterpene Indole Alkaloids (MIA). A practical single-step procedure to assemble strictosidine from secologanin is described via a bioinspired Pictet–Spen...

    Yingchao Dou, Laurent Evanno, Erwan Poupon, Guillaume Vincent in Catharanthus roseus (2022)

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    Protocol

    Microtubule Reorganization During ABA-Induced Stomatal Closure in Arabidopsis

    Plants must cope with diverse environmental stresses during growth and development, among which drought is one of the most concerning global threats. Recent studies have shown that the disassembly of the micro...

    Liru Dou, **angfeng Wang, Tonglin Mao in The Plant Cytoskeleton (2023)