![Loading...](https://link.springer.com/static/c4a417b97a76cc2980e3c25e2271af3129e08bbe/images/pdf-preview/spacer.gif)
-
Protocol
Convenient and Efficient Enrichment of the CD133+ Liver Cells from Rat Fetal Liver as a Source of Liver Stem/Progenitor Cells
Although stem cells are commonly isolated by fluorescence-activated cell sorting or magnetic affinity cell sorting, they are very expensive, and they need known markers. However, there is no specific marker fo...
-
Protocol
Prediction of Protein Phosphorylation Sites by Integrating Secondary Structure Information and Other One-Dimensional Structural Properties
Studies on phosphorylation are important but challenging for both wet-bench experiments and computational studies, and accurate non-kinase-specific prediction tools are highly desirable for whole-genome annota...
-
Protocol
Experimentally Validated Plant lncRNAs in EVLncRNAs Database
Plant long noncoding RNAs (lncRNAs) play important functional roles in various biological processes. Most databases deposit all plant lncRNA candidates produced by high-throughput experimental and/or computati...
-
Protocol
Pictet–Spengler Reaction for the Chemical Synthesis of Strictosidine
Strictosidine is the common biosynthetic precursor of Monoterpene Indole Alkaloids (MIA). A practical single-step procedure to assemble strictosidine from secologanin is described via a bioinspired Pictet–Spen...
-
Protocol
Microtubule Reorganization During ABA-Induced Stomatal Closure in Arabidopsis
Plants must cope with diverse environmental stresses during growth and development, among which drought is one of the most concerning global threats. Recent studies have shown that the disassembly of the micro...