10 Result(s)
within
Life Sciences
Cell Biology
Cell Culture
**li Li
Protocol
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Protocol
Mouse Epidermal Keratinocyte Culture
Cultured mouse epidermal keratinocytes provide a powerful model for studying epidermal proliferation, differentiation, pathogenesis, and oncogenic transformation. Primary mouse keratinocytes can be isolated fr...
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Isolation and Cultivation of Dermal Stem Cells that Differentiate into Functional Epidermal Melanocytes
Human melanocytes have been extensively studied, but a melanocyte stem cell reservoir in glabrous skin has not yet been found. Human dermis contains cells that are nonpigmented but can differentiate to several...
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Exploiting the Liberation of Zn2+ to Measure Cell Viability
Zn2+ ions are a critical component of cellular machinery. The ion is required for the function of many cell components crucial to survival, such as transcription factors, protein synthetic machinery, metabolic en...
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Isolation and Culture of Embryonic Pancreas and Liver
Culturing embryonic tissue in an in vitro setting offers the unique ability to manipulate the external medium and therefore to investigate the pathways involved in regulating normal organogenesis as well as pr...
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Optimization of Physiological Xenofree Molecularly Defined Media and Matrices to Maintain Human Embryonic Stem Cell Pluripotency
We describe in this chapter the development of a xenofree molecularly defined medium, SBX, associated with xenofree matrices, to maintain human embryonic stem cell (hESC) pluripotency as determined by phenotyp...
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Functional Assays for Hematopoietic Stem Cell Self-Renewal
Stem cells are defined by the ability to self-renew. Specific functional assays have been developed for the rigorous identification and quantification of hematopoietic stem cells (HSCs), making these cells the...
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Isolation and Culture of Adult Mouse Hepatocytes
The liver performs a multitude of functions including the regulation of carbohydrate, fat, and protein metabolism, the detoxification of endo- and xenobiotics, and the synthesis and secretion of plasma protein...
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Generation of Novel Rat and Human Pluripotent Stem Cells by Reprogramming and Chemical Approaches
Although embryonic stem cells (ESCs) have been established from mice since 1981, attempts to derive its counterparts from various other mammals, including rats, have not succeeded. Recently, induced pluripoten...
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Culture of Bone Marrow Mesenchymal Stem Cells on Engineered Matrix
Mesenchymal stem cells (MSCs) have huge potential to be used in cell therapies because of their pluripotency and immunoregulatory attributes. To harness and maximize the therapeutic potential of MSCs, a thorou...
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In Vitro Differentiation of Neural Precursors From Human Embryonic Stem Cells
We describe a procedure for efficient and reproducible differentiation of neuroectodermal cells from human embryonic stem (ES) cells using an adherent colony culture. ES cell colonies are detached intact from ...
