22 Result(s)
within
Protocol
**ao**g Mu
-
Protocol
Stable Discoidal Bicelles: Formulation, Characterization, and Functions
Bicellar mixtures have been used as alignable membrane substrates under a magnetic field applicable for the structural characterization of membrane-associated proteins. Recently, it has shown that bicelles can...
-
Protocol
Quantifying Social Interactions in Medaka Fish
Zebrafish (Danio rerio) and medaka fish (Oryzias latipes) are popular vertebrate models for various biology fields. Medaka fish have recently attracted attention in the field of social neuroscience due to some of...
-
Protocol
Modification of Chloroplast Antioxidant Capacity by Plastid Transformation
As immobile organisms, green plants must be frequently challenged by a broad range of environmental stresses. During these constantly adverse conditions, reactive oxygen species (ROS) levels can rise extremely...
-
Protocol
Ketamine and Other Glutamate Receptor Antagonists As Fast-Actin
The glutamate hypothesis of depression was prominently emerging in this decade. Evidence has shown that low-dose ketamine infusion or intranasal S-ketamine spray exhibited a prominent and rapid antidepressant ...
-
Protocol
First-Order Correction of Statistical Significance for Screening Two-Way Epistatic Interactions
If one uses data to identify the most likely epistatic interaction between two genetic units, and then tests if the identified interaction is associated with a phenotype, the nominal statistical evidence will ...
-
Protocol
CRISPR-Cas12a-Based DNA Detection for Fast Pathogen Diagnosis and GMO Test in Plants
DNA test is widely used in plant pathogen diagnosis and genetically modified organism (GMO) administration. To date, a low-cost, user-friendly, and field-deployable DNA test method with high accuracy and sensi...
-
Protocol
In Vitro Methods to Evaluate the Effects of Mesenchymal Stem Cells on TGF-β1-Induced Pulmonary Fibrosis
A co-culture model of mesenchymal stem cells (MSCs) and fibroblasts is an efficient and rapid method to evaluate the anti-fibrotic effects of MSCs-based cell therapy. Transforming growth factor (TGF)-β1 plays ...
-
Protocol
Detection of Long Non-coding RNA Expression by Non-radioactive Northern Blots
With the advances in sequencing technology and transcriptome analysis, it is estimated that up to 75% of the human genome is transcribed into RNAs. This finding prompted intensive investigations on the biologi...
-
Protocol
Detection of Post-translational Modifications on MYC
Detection of post-translational modifications in c-Myc is an invaluable tool in assessing Myc status, particularly in cancer. However, it can be challenging to detect these modifications. The evaluation of s...
-
Protocol
Methods for the Study of Long Noncoding RNA in Cancer Cell Signaling
With the advances in sequencing technology and transcriptome analysis, it is estimated that up to 75% of the human genome is transcribed into RNAs. This finding prompted intensive investigations on the biologi...
-
Protocol
The Role of RNA Editing in the Immune Response
The innate immune receptors in higher organisms have evolved to detect molecular signatures associated with pathogenic infection and trigger appropriate immune response. One common class of molecules utilized ...
-
Protocol
Stable Discoidal Bicelles: A Platform of Lipid Nanocarriers for Cellular Delivery
Bicellar mixtures have been used as alignable membrane substrates for the structural characterization of membrane-associated proteins. Most recently, it has been shown that bicelles can serve as nanocarriers t...
-
Protocol
Silicon Nanoparticles and Microparticles
Porous silicon nanoparticles and microparticles have great potential as drug delivery carriers of a variety of therapeutic agents in chemotherapeutics, gene delivery, molecular diagnostics, and immunotherapy d...
-
Protocol
Separation of Amino Acids by Capillary Electrophoresis with Light-Emitting Diode-Induced Fluorescence in the Presence of Electroosmotic Flow
In this chapter, we describe a method to identify amino acids (AA) by capillary electrophoresis in conjunction with light-emitting diode-induced fluorescence (LEDIF). First, amino acids labeled with naphthalen...
-
Protocol
Mass Spectrometry for Protein Quantification in Biomarker Discovery
Major technological advances have made proteomics an extremely active field for biomarker discovery in recent years due primarily to the development of newer mass spectrometric technologies and the explosion i...
-
Protocol
Molecular Methods for Studying the Cryphonectria parasitica – Hypovirus Experimental System
The interaction of the filamentous fungal plant pathogen Cryphonectria parasitica with its virulence-attenuating viruses provides a unique platform to explore the molecular biology and genetics of virus–host inte...
-
Protocol
Real-Time Monitoring of Cellular Responses to Carbon Nanotubes
Dynamic cellular responses to carbon nanotubes were monitored by a real-time cell electronic sensing assay. This approach is based on the parallel impedance measurement of attached cells using electronic sens...
-
Protocol
Preventing Immune Rejection Through Gene Silencing
Dendritic cells (DCs) comprise a family of professional antigen-presenting cells responsible for the induction of primary immune responses. DCs are also important for the induction of immunological tolerance. ...
-
Protocol
Label-Free Mass Spectrometry-Based Protein Quantification Technologies in Protein Biomarker Discovery
Major technological advances have made proteomics an extremely active field for biomarker discovery in recent years due primarily to the development of newer mass spectrometric technologies and the explosion i...
-
Protocol
Small-Angle Neutron Scattering to Detect Rafts and Lipid Domains
The detection and characterization of lateral heterogeneities or domains in lipid mixtures has attracted considerable interest, because of the roles that such domains may play in biological function. Studies o...
