12 Result(s)
within
Protocol
**-Peng Li
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Protocol
Proteomic Profiling of Cerebrospinal Fluid by 16-Plex TMT-Based Mass Spectrometry
Mass spectrometry (MS) has become a mainstream platform for comprehensive profiling of proteome, especially with the improvement of multiplexed tandem mass tag labeling coupled with two-dimensional liquid chro...
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Protocol
Genetic Studies of Natural Glycosphingolipid Ligands for NKT Cells
Glycosphingolipids (GSL) are natural ligands of NKT cells. Several laboratories have reported the in vitro activity of isoglobotriosylceramide (iGb3) in stimulating NKT cells. However, the knockout mice of iGb...
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Protocol
Quantifying Proteome and Protein Modifications in Activated T Cells by Multiplexed Isobaric Labeling Mass Spectrometry
The dynamic regulation of protein function by altered protein expression and post-translational modifications (PTMs) is essential for T cell function, but it has remained difficult to systemically quantify suc...
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Protocol
Characterization of the Role of Rab18 in Mediating LD–ER Contact and LD Growth
Lipid droplets (LDs) are dynamic cellular organelles found in most eukaryotic cells. Lipid incorporation from endoplasmic reticulum (ER) to LD is important in controlling LD growth and intracellular lipid home...
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Protocol
Identification of AI-2 Quorum Sensing Inhibitors in Vibrio harveyi Through Structure-Based Virtual Screening
Quorum sensing (QS) is a cell-to-cell communication system that regulates gene expression as a result of the production and perception of signal molecules called autoinducers (AIs). AI-2 is a QS autoinducer pr...
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Protocol
Analysis of Peroxisome Biogenesis in Pollen by Confocal Microscopy and Transmission Electron Microscopy
Peroxisome is an essential single-membrane bound organelle in most eukaryotic cells and functions in diverse cellular processes. De novo formation, division, and turnover of peroxisomes contribute to its bioge...
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Protocol
Chronic Myeloid Leukemia (CML) Mouse Model in Translational Research
Chronic myeloid leukemia (CML) is a myeloproliferative disorder characterized by increased proliferation of granulocytic cells without the loss of their capability to differentiate. CML is a clonal disease, or...
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Protocol
Stimulation of Cerebral Angiogenesis by Gene Delivery
Angiogenesis, an important process for long term neurological recovery, could be induced by ischemic brain injury. In this chapter, we describe a system to deliver adeno-associated viral (AAV) vector-mediated ...
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Protocol
In Vitro Reconstitution of Escherichia coli O86 O Antigen Repeating Unit
Polysaccharides constitute a major component of the bacterial cell surface. They play critical roles in the interactions between bacteria and the host environments, and consequently contribute to the virulence...
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Protocol
CML Mouse Model in Translational Research
Chronic myeloid leukemia (CML) is a myeloproliferative disorder characterized by increased proliferation of granulocytic cells without the loss of their capability to differentiate. CML is derived from the hem...
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Protocol
Synthetic Fluorescent Probes for Imaging of Peroxynitrite and Hypochlorous Acid in Living Cells
Peroxynitrite (ONOO–) and hypochlorous acid (HOCl) are two highly reactive oxygen species generated in biological systems. The overproduction of peroxynitrite or hypochlorous acid is implicated in a broad array o...
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Protocol
Synthesis of Complex Carbohydrates and Glyconjugates
The lipopolysaccharide of capsule-deficient Haemophilus infuenzae strain Rd contains an N-acetylgalactosamine residue attached to the terminal globotriose moiety in the Hex5 glycoform. Genome analysis identified ...
