6 Result(s)
within
Protocol
**-Long **ao
Cell Biology
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Protocol
Fluorescent In Situ Hybridization of Nuclear Bodies in Drosophila melanogaster Ovaries
Fluorescent in situ hybridization (FISH) is a technique for determining the cytological localization of RNA or DNA molecules. There are many approaches available for generating in situ hybridization probes and...
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Protocol
Assessing Reprogramming by Chimera Formation and Tetraploid Complementation
Pluripotent stem cells can be evaluated by pluripotent markers expression, embryoid body aggregation, teratoma formation, chimera contribution and even more, tetraploid complementation. Whether iPS cells in ge...
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Protocol
Visualizing Cytoophidia Expression in Drosophila Follicle Cells via Immunohistochemistry
We describe a user-friendly immunohistochemical approach for the detection of protein localization in Drosophila ovaries, here focusing on CTP synthase. This approach mainly uses fluorescently labeled antibodies ...
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Protocol
Imaging Calcium Sparks in Cardiac Myocytes
Calcium ions play fundamental roles in many cellular processes in virtually all type of cells. The use of Ca2+ sensitive fluorescent indicators has proven to be an indispensable tool for studying the spatio-tempo...
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Protocol
Monitoring the Temporal and Spatial Distribution of RNA in Living Yeast Cells
RNA localization is a cellular process to spatially restrict translation of specific proteins to defined regions within or between cells. Most localized mRNAs contain cis-acting localization elements in the 3′-un...
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Protocol
Derivation and Propagation of Embryonic Stem Cells in Serum- and Feeder-Free Culture
The availability of murine embryonic stem (ES) cells has revolutionized the study of mammalian development and disease. We recently developed a culture medium that has enabled us to identify the essential sign...
