8 Result(s)
within
Protocol
** Young Lee
-
Protocol
Extraction and Upconcentration of Adsorbates from Precisely Defined Area for Quantitative MALDI Mass Spectrometry Imaging
Mass spectrometry imaging (MSI) allows label-free detection of a wide range of biomolecules and simultaneously provides their spatial distributions. In particular, MSI by matrix-assisted laser desorption/ioniz...
-
Protocol
Multiplexed Tissue Tomography
Multiplexed tissue tomography enables comprehensive spatial analysis of markers within a whole tissue or thick tissue section. Clearing agents are often used to make tissue transparent and facilitate deep tiss...
-
Protocol
Central and Peripheral Secondary Cell Death Processes after Transient Global Ischemia in Nonhuman Primate Cerebellum and Heart
Cerebral ischemia and its pathological sequelae are responsible for severe neurological deficits generally attributed to the neural death within the infarcted tissue and adjacent regions. Distal brain regions,...
-
Protocol
Histopathological and Behavioral Assessments of Aging Effects on Stem Cell Transplants in an Experimental Traumatic Brain Injury
Traumatic brain injury (TBI) displays cognitive and motor symptoms following the initial injury which can be exacerbated by secondary cell death. Aging contributes significantly to the morbidity of TBI, with h...
-
Protocol
Effects of Sterols on the Development and Aging of Caenorhabditis elegans
Although Caenorhabditis elegans lacks several components of the de novo sterol biosynthetic pathway, it requires sterols as essential nutrients. Supplemental cholesterol undergoes extensive enzymatic modification...
-
Protocol
Proteomic Analysis of the Sterol-Mediated Signaling Pathway in Caenorhabditis elegans
Since Caenorhabditis elegans is incapable of de novo cholesterol biosynthesis, it must utilize other nonpermissive sterols that are present in the environment by converting them into cholesterol for cellular func...
-
Protocol
Protein Profiling of Human Plasma Samples by Two-Dimensional Electrophoresis
Human plasma is regarded the most complex and well-known clinical specimen that can be easily obtained; alterations in the levels of plasma proteins or their corresponding enzyme activities may reflect either ...
-
Protocol
Overview and Introduction to Clinical Proteomics
As the field of clinical proteomics progresses, discovery of disease biomarkers becomes paramount. However, the immediate challenges are to establish standard operating procedures for both clinical specimen ha...
