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Protocol
A Protocol for the Plasma Membrane Proteome Analysis of Rice Leaves
Subcellular proteome analysis is one of the most effective ways to reduce the complexity of total proteome. With the advancement in protein extraction methodologies, it is now possible to fractionate and isola...
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Protocol
Application of an (18-Crown-6)-2,3,11,12-Tetracarboxylic Acid-Based Chiral Stationary Phase in Capillary Electrochromatography
Capillary electrochromatography is employed for the enantioseparation of α-amino acids and their derivatives. The synthesis and application of a covalently bonded chiral stationary phase containing (−)-(18-cro...
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Protocol
Measurement of the Multiple Activities of 26S Proteasomes
Because proteasomes catalyze most of the protein degradation in mammalian cells, and their functioning is essential for cellular homeostasis, proteasome structure, biochemical mechanisms, and regulation in nor...
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Protocol
Depletion of RuBisCO Protein Using the Protamine Sulfate Precipitation Method
Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) is a major high-abundant protein (HAP) in the plant leaves which hinders analysis of low-abundant proteins (LAP). In this chapter, we describe a highly...
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Protocol
Differential Proteome and Secretome Analysis During Rice–Pathogen Interaction
Substantial evidences implicate that sample preparation and protein extraction in proteomic studies of plant–pathogen interactions are critical to understand cross talk between host and pathogen. Therefore, in...
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Protocol
Rice Proteomic Analysis: Sample Preparation for Protein Identification
Rice is one of the most important food and cereal crop plants in the world. Rice proteomics began in the 1990s. Since then, considerable progress has been made in establishing protocols from isolation of rice ...
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Protocol
S5a/Rpn10, a UIM-Protein, as a Universal Substrate for Ubiquitination
The assay of the activity of ubiquitin (Ub) ligases (E3s) and screens for pharmacological agents that alter their function are a continual challenge for basic investigators as well as in drug development. The ...
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Protocol
Formation of Nondegradable Forked Ubiquitin Conjugates by Ring-Finger Ligases and Its Prevention by S5a
The biological role and fates of ubiquitin (Ub) conjugates are determined by the nature of the ubiquitin chain formed on the protein. Recently, we reported that Ring-finger and U-box ubiquitin ligases (E3s), w...
