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Chapter
Site-Directed Mutagenesis of Residues in the Active Site of Sepiapterin Reductase
Tetrahydrobiopterin (BH4) is the natural cofactor for producing neurotransmitters such as catecholamines, indoleamines, and nitric oxide. Sepiapterin reductase (SPR; EC 1. 1. 1. 153) catalyzes the terminal step i...
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Chapter
Structure and Function of Subtilisin BPN’ as Studied Through Crystallographic Studies on a Series of its Complexes with Genetically Engineered Proteinaceous Inhibitor SSI
Streptomyces subtilisin inhibitor (SSI) is one of the few well-characterized microbial proteinase inhibitors and is a stable dimer (I2) composed of two identical subunits, each of molecular weight 1 1,5001. It st...