8 Result(s)
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Chapter
Stanley G. Sawicki
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Chapter
Coronavirus Genome Replication
Viruses belonging to the family Coronaviridae are unique among RNA viruses because of the unusually large size of their genome, which is of messenger- or positive- or plus-sense. It is ∼30,000 bases or 2–3 tim...
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Mouse Hepatitis Virus Minus-strand Templates are Unstable and Turnover During Viral Replication
During the first 6 hours after infection at 37°C, MHV-A59 infected cells accumulate seven species of minus strands, one equal in length to the viral genome RNA and six that are subgenomic and with sizes equal ...
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Chromatography of Mouse Hepatitis Virus Replicative Intermediate and Replicative Form RNA
One of our goals is to obtain large amounts of selected size classes of the Coronavirus replicative intermediates (RI) and native replicative form (RF) RNA that contain the templates for viral genome and messe...
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Selection in Persistently Infected Murine Cells of an MHV-A59 Variant with Extended Host Range
Murine Coronavirus MHV-A59 normally infects only murine cells in vitro and causes transmissible infection only in mice. In the 17 Cl 1 line of murine cells, the receptor for MHV-A59 is MHVR, a biliary glycoprotei...
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Role of the Nonstructural Polyproteins in Alphavirus RNA Synthesis
Alphaviruses are of general interest because they replicate in such a broad range of animals, including invertebrates as well as mammals and birds. This broad replicative potential is an essential part of thei...
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Coronaviruses use Discontinuous Extension for Synthesis of Subgenome-Length Negative Strands
We have developed a new model for coronavirus transcription, which we call discontinuous extension, to explain how subgenome-length negatives stands are derived directly from the genome. The current model call...
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Characterization of a Small Plaque Mutant of the A59 Strain of Mouse Hepatitis Virus Defective in Cell Fusion
The virions of murine coronaviruses contain nucleocapsid protein and two membrane associated proteins E1 and E2. E2 or S glycoprotein forms the peplomer or spike protein and, as shown by Lawrence Sturman and K...
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Third International Congress of Histochemistry and Cytochemistry
The tissue distribution of enzymatic activities in intestinal metaplasia stomachs exhibiting chronic gastritis was compared histochemically with that of the small intestine in man.
