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244 Result(s)
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Chapter
Chemical Probes for the Geometry of Membrane Proteins
The position of the protein components of biological membranes both perpendicular to and in the plane of the permeability barrier has been the subject of much recent interest. The “sidedness” of the identifiab...
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Chapter and Conference Paper
Regeneration of virus-free plants through in vitro culture
This article deals with the theories, methodology and practices of in vitro virus elimination. Viruses may be eradicated from infected stocks through meristem or callus cultures due to the low titre, or even abse...
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Chapter
Isolation and Characterization of a Growth Regulatory Factor from 3T3 Cells
The mouse fibroblast line 3T3 (1) exhibits a form of growth control in vitro in that it reaches only a very low saturation density and can remain for long periods of time in a viable but nondividing state. Thi...
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Chapter and Conference Paper
Physiological and Biochemical Aspects of Mammalian Hibernation
Hibernation in mammals in response to cold and food shortage in winter is characterized by a profound reduction in metabolism (to 1% or less of normal) and body temperature (to near 0 °C) which may last from a...
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Chapter and Conference Paper
Primate Status and Conservation in China
Sixteen species or 25 subspecies of primates representing three families and six genera are recorded in China (Quan et al., 1981a, 1981b; Zhang et al., 1981; Honacki et al., 1982; Li and Lin, 1983; Fooden et a...
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Chapter
Dynamic Interactions of the Second Messenger Systems
The response of living cells to changes in cell environment depends on the production and actions of second messenger molecules. The two most extensively studied second messengers are Ca2+ and cAMP, each of which...
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Chapter
Modification of Leucyl-tRNA Synthetase by Affinity Labeling and Limited Proteolysis
Aminoacyl-tRNA synthetases catalyses catalyze the transfer of amino acid to its cognate tRNA.
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Chapter
Roles for Plasma Membrane Redox Systems in Cell Growth
Correlative evidence from cultured mammalian cells suggests that growth and plasma membrane redox activities are somehow related. This comes in part from studies with hormones, growth factors and anticancer dr...
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Chapter and Conference Paper
The Three-Dimensional Structure of Bacterial Surface Layers
The research of bacterial surface layers (S-layers) is in the unusual situation that more is known about the structure than about the function of S-layers. While it has been argued that we must first understan...
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Chapter
Laser Raman Spectroscopy of Nucleic Acids
Recent applications of the technique of single crystal X-ray diffraction, in combination with structure refinement methods, have revealed architectural details of right- and left-handed DNA helices at atomic r...
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Chapter
Unusual DNA Structures and the Probes Used for their Detection
A veritable explosion in our knowledge of unusual DNA structures has occurred in recent years. Our enhanced knowledge about specific DNA sequences via DNA sequencing and cloning has been a contributory factor....
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Chapter
Reinvestigation on BNPS-Skatole Cleavage of Tryptophan Polypeptides and the C-Terminal Sequencing of the Cleavage Fragments
In the light of indole chemistry Patchornik and Witkop (1960) have proposed the mechanism of oxidative cleavage of tryptophan polypeptides and dioxyindolylalanine spiro lactone (Dial) as the oxidation product ...
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Chapter and Conference Paper
Regulation of the Rat Insulin II Gene: cis- and trans-acting Factors
Insulin is one of the key peptide hormones in the control of growth and metabolism. The imbalance of insulin action results in diabetes mellitus which has broad impact on the society. Aside from its medical im...
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Chapter
Catalysis by Isomeric Forms of Covalently Labeled F1-ATPase
When bovine heart mitochondrial F1-ATPase was labeled with low concentrations of NBD-Cl* in the dark in the presence of ATP at pH 7, the NBD-label was almost exclusively attached to a specific tyrosine residue un...
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Chapter and Conference Paper
Regulation of Glucocorticoid Receptor Protein and Gene Expression by Glucocorticoids
Glucocorticoids, members of the highly conserved steroid hormone family in evolution, exert numerous physiological effects on the developmental and adaptational processes of eukaryotic organisms. In general, f...
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Chapter
Anther Culture 28 — A New Disease-Resistant and High-Yielding Variety of Winter Wheat
Only three generations were required for Anther Culture 28 (An.Cul.28) — a new disease-resistant and high-yielding variety of winter wheat — to be formed by anther culture. An.Cul.28 not only shows stability o...
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Chapter
Effects of a DNA Intercalator, Ethidium Bromide on Chromatin Structure of Chicken Erythrocyte
DNase I hypersensitive sites (DHS) are the most loosely packed regions in chromatin, which are ultrasensitive, typically 100 times more than bulk chromatin, to DNase I as well as other DNA cleaving agents incl...
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Chapter and Conference Paper
Molecular Analysis of Pathogenesis in Ustilago maydis
We are isolating and studying genes required for pathogenicity of Ustilago maydis, the causative agent of corn smut disease (Christensen, 1963). This phytopathogenic basidiomycete offers an attractive system in w...
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Chapter and Conference Paper
Recent Progress on Understanding the Initiation of Translation in the Chloroplasts of Euglena Gracilis
The mechanism of protein biosynthesis in chloroplasts is currently not well understood. However, in general, the process of translation in this organelle is thought to resemble that in prokaryotes more closely...
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Chapter and Conference Paper
Evidence for three Phytochromes in Avena
Shortly after the identification of phytochrome as a chromoprotein, Butler and Lane (1965) raised the question of whether the ‘bulk’ phytochrome detected in etiolated seedlings was physiologically functional, ...