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Open AccessA classical swine fever virus E2 fusion protein produced in plants elicits a neutralizing humoral immune response in mice and pigs
Classical swine fever (CSF) is one of the most important viral diseases of swine worldwide. Although live or attenuated virus vaccines have been used to control CSFV, it is difficult to distinguish vaccinated ...
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Article
Application of recombinant hemagglutinin proteins as alternative antigen standards for pandemic influenza vaccines
The single radial immunodiffusion (SRID) assay, used to quantify hemagglutinin (HA) in influenza vaccines, requires reference reagents; however, because centralized production of reference reagents may slow th...
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Article
Optimization of enantioselective synthesis of methyl (R)-2-chloromandelate by whole cells of Saccharomyces cerevisiae
Methyl (R)-2-chloromandelate, a key intermediate in the synthesis of clopidogrel, was obtained by the reduction of methyl-2-chlorobenzoylformate using whole cells of Saccharomyces cerevisiae. A 100% conversion an...
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Article
Development of 3-D poly(trimethylenecarbonate-co-ε-caprolactone)-block-poly(p-dioxanone) scaffold for bone regeneration with high porosity using a wet electrospinning method
The three dimensional (3-D) poly(trimethylenecarbonate-co-ε-caprolactone)-block-poly(p-dioxanone) scaffold was made using a wet electrospinning method and its application as a tissue engineered matrix was evaluat...
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Article
1,3-Dichloro-2-propanol induces apoptosis via both calcium and ROS in mouse melanoma cells
We demonstrated that the apoptotic cell death of mouse melanoma cells exposed to 1,3-dichloro-2-propanol (DCP) was Ca2+ dependent. 1,3-DCP inhibited the growth of mouse melanoma cells in a concentration-dependent...
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Article
Development of 3-D nanofibrous fibroin scaffold with high porosity by electrospinning: implications for bone regeneration
We made a three-dimensional (3-D) nanofibrous fibroin scaffold (NFS) with high porosity (94%) and examined its feasibility in bone regeneration. Under scanning electron microscopy, MC3T3-E1 preosteoblasts on t...
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Article
Intracellular drug delivery using poly(d,l-lactide-co-glycolide) nano- particles derivatized with a peptide from a transcriptional activator protein of HIV-1
Biodegradable poly(d,l-lactide-co-glycolide) (PLGA) nanoparticles were derivatized with Tat49−57 peptide, which is the protein transduction domain from the transcriptional activator Tat protein of human immunodef...
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Article
Removal of attenuator region of thr operon increases the production of threonine in Escherichia coli
Expression of the threonine (thr) operon of Escherichia coli is regulated by an attenuation mechanism. The regulatory portion of the thr operon contains a region coding for leader peptide that includes both conse...
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Article
Enhanced specific antibody productivity of hybridomas resulting from hyperosmotic stress is cell line-specific
Hybridomas with non-growth-associated antibody production are thought to exhibit enhanced specific monoclonal antibody productivity (q MAb) when subjected to hyperosmotic stress. Two hybridoma cell lines exhibiti...