19 Result(s)
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Life Sciences
Biotechnology
Microbiology
You** Park
Article
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Article
Production of anthraquinones from cell and organ cultures of Morinda species
Since ancient times, Morinda species, particularly Morinda citrifolia, have been used for their therapeutic benefits. Iridoids, anthraquinones, coumarins, flavonoids, lignans, phytosterols, and carotenoids are ex...
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Optimal β-galactosidases for producing high-titer 3,6-anhydro-L-galactose from red-algal agarobiose
3,6-Anhydro-L-galactose (L-AHG) is a monomeric sugar in agarose derived from red macroalgae. Owing to its various physiological activities such as anti-inflammation, moisturizing, skin whitening, anti-colon ca...
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Streptococcus ruminicola sp. nov., new species of the Streptococcus bovis/Streptococcus equinus complex (SBSEC) isolated from the rumen of Korean domestic ruminants
A total of three Gram-positive, and oxidase and catalase-negative facultative anaerobic non-motile bacteria were isolated from the rumen fluid of cows and goats and these strains were designated CNU_G2T, CNU_77-6...
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Cyclo(Phe-Pro) produced by Vibrio species passes through biological membranes by simple diffusion
Cyclo(Phe-Pro) (cFP), produced by the Vibrio species, plays the dual roles of being a signaling molecule and a virulence factor. Acting modes of this compound have recently been characterized at the molecular lev...
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Open AccessA classical swine fever virus E2 fusion protein produced in plants elicits a neutralizing humoral immune response in mice and pigs
Classical swine fever (CSF) is one of the most important viral diseases of swine worldwide. Although live or attenuated virus vaccines have been used to control CSFV, it is difficult to distinguish vaccinated ...
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Open AccessIncreased resistance of a methicillin-resistant Staphylococcus aureus Δagr mutant with modified control in fatty acid metabolism
Methicillin-resistant Staphylococcus aureus (MRSA) strains are distinct from general Staphylococcus strains with respect to the composition of the membrane, ability to form a thicker biofilm, and, importantly, ab...
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Application of recombinant hemagglutinin proteins as alternative antigen standards for pandemic influenza vaccines
The single radial immunodiffusion (SRID) assay, used to quantify hemagglutinin (HA) in influenza vaccines, requires reference reagents; however, because centralized production of reference reagents may slow th...
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Microbial arsenite oxidation with oxygen, nitrate, or an electrode as the sole electron acceptor
The purpose of this study was to identify bacteria that can perform As(III) oxidation for environmental bioremediation. Two bacterial strains, named JHS3 and JHW3, which can autotrophically oxidize As(III)–As(...
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PCE dechlorination by non-Dehalococcoides in a microbial electrochemical system
The bioremediation of tetrachloroethene (perchloroethene; PCE) contaminated sites generally requires a supply of some fermentable organic substrates as an electron donor. On the other hand, organic substrates ...
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Effect of the cathode potential and sulfate ions on nitrate reduction in a microbial electrochemical denitrification system
Recently, bioelectrochemical systems have been demonstrated as advantageous for denitrification. Here, we investigated the nitrate reduction rate and bacterial community on cathodes at different cathode potent...
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Electron flux and microbial community in microbial fuel cells (open-circuit and closed-circuit modes) and fermentation
A closed-circuit microbial fuel cell (C-MFC) was operated to investigate the electron flux under fed-batch mode, and the results were compared to those of open-circuit MFC (O-MFC) and a fermentation reactor (F...
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Optimization of enantioselective synthesis of methyl (R)-2-chloromandelate by whole cells of Saccharomyces cerevisiae
Methyl (R)-2-chloromandelate, a key intermediate in the synthesis of clopidogrel, was obtained by the reduction of methyl-2-chlorobenzoylformate using whole cells of Saccharomyces cerevisiae. A 100% conversion an...
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Development of 3-D poly(trimethylenecarbonate-co-ε-caprolactone)-block-poly(p-dioxanone) scaffold for bone regeneration with high porosity using a wet electrospinning method
The three dimensional (3-D) poly(trimethylenecarbonate-co-ε-caprolactone)-block-poly(p-dioxanone) scaffold was made using a wet electrospinning method and its application as a tissue engineered matrix was evaluat...
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Facilitation of polymerase chain reaction with thermostable inorganic pyrophosphatase from hyperthermophilic archaeon Pyrococcus horikoshii
An inorganic pyrophosphatase (PPases) was cloned from the hyperthermophilic archaeon Pyrococcus horikoshii and was expressed in and purified from Escherichia coli. The recombinant inorganic pyrophosphatase (PhPPa...
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1,3-Dichloro-2-propanol induces apoptosis via both calcium and ROS in mouse melanoma cells
We demonstrated that the apoptotic cell death of mouse melanoma cells exposed to 1,3-dichloro-2-propanol (DCP) was Ca2+ dependent. 1,3-DCP inhibited the growth of mouse melanoma cells in a concentration-dependent...
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Development of 3-D nanofibrous fibroin scaffold with high porosity by electrospinning: implications for bone regeneration
We made a three-dimensional (3-D) nanofibrous fibroin scaffold (NFS) with high porosity (94%) and examined its feasibility in bone regeneration. Under scanning electron microscopy, MC3T3-E1 preosteoblasts on t...
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Intracellular drug delivery using poly(d,l-lactide-co-glycolide) nano- particles derivatized with a peptide from a transcriptional activator protein of HIV-1
Biodegradable poly(d,l-lactide-co-glycolide) (PLGA) nanoparticles were derivatized with Tat49−57 peptide, which is the protein transduction domain from the transcriptional activator Tat protein of human immunodef...
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Removal of attenuator region of thr operon increases the production of threonine in Escherichia coli
Expression of the threonine (thr) operon of Escherichia coli is regulated by an attenuation mechanism. The regulatory portion of the thr operon contains a region coding for leader peptide that includes both conse...
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Enhanced specific antibody productivity of hybridomas resulting from hyperosmotic stress is cell line-specific
Hybridomas with non-growth-associated antibody production are thought to exhibit enhanced specific monoclonal antibody productivity (q MAb) when subjected to hyperosmotic stress. Two hybridoma cell lines exhibiti...
