Page
%P
2 Result(s)
-
Article
Open AccessAFEAP cloning: a precise and efficient method for large DNA sequence assembly
Recent development of DNA assembly technologies has spurred myriad advances in synthetic biology, but new tools are always required for complicated scenarios. Here, we have developed an alternative DNA assembl...
-
Article
Open AccessA restriction-free method for gene reconstitution using two single-primer PCRs in parallel to generate compatible cohesive ends
Restriction-free (RF) cloning, a PCR-based method for the creation of custom DNA plasmids, allows for the insertion of any sequence into any plasmid vector at any desired position, independent of restriction s...
