22 Result(s)
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Article
So young Park
Biotechnology
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Article
Production of anthraquinones from cell and organ cultures of Morinda species
Since ancient times, Morinda species, particularly Morinda citrifolia, have been used for their therapeutic benefits. Iridoids, anthraquinones, coumarins, flavonoids, lignans, phytosterols, and carotenoids are ex...
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Optimal β-galactosidases for producing high-titer 3,6-anhydro-L-galactose from red-algal agarobiose
3,6-Anhydro-L-galactose (L-AHG) is a monomeric sugar in agarose derived from red macroalgae. Owing to its various physiological activities such as anti-inflammation, moisturizing, skin whitening, anti-colon ca...
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Establishment of bioreactor cultures for the production of chlorogenic acid and ferulic acid from adventitious roots by optimization of culture conditions in Angelica acutiloba (Siebold & Zucc.) Kitag
Adventitious roots were induced from root explants of Angelica acutilobaand suspension cultures were established in shake flasks. The effect of different concentrations of indole acetic acid (IBA, 0–38.4 µM), the...
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Brazzein protein production in transgenic carrot cells using air-lift bioreactor culture
Recombinant-protein production in plants is a more effective system than the mammalian cell system because it can be more easily scaled up, has a low production cost, and is animal pathogen free. In this study...
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Microfluidic Single-cell Trap** and Cultivation for the Analysis of Host-viral Interactions
The isolation of single cells and their further cultivation in confined chambers are essential to the collection of statistically reliable temporal information in cell-based biological experiments. In this wor...
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Open AccessLarge-scale production of recombinant miraculin protein in transgenic carrot callus suspension cultures using air-lift bioreactors
Miraculin, derived from the miracle fruit (Synsepalum dulcificum), is a taste-regulating protein that interacts with human sweet-taste receptors and transforms sourness into sweet taste. Since miracle fruit is cu...
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Increased brazzein expression by abiotic stress and bioreactor culture system for the production of sweet protein, brazzein
Plant-based recombinant protein production systems are powerful tools that can be used instead of microbial and animal cell culture systems. The aim of this study was to establish a stable brazzein production ...
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Effect of nutritional factors on the accretion of secondary metabolites in Malaysian ginseng adventitious root cultures
In this study, we aimed to verify the effect of nutritional factors on the accretion of secondary metabolites in the adventitious root (AR) cultures of Malaysian ginseng (Eurycoma longifolia Jack) grown in small-...
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Enhanced biosynthesis of triterpenoids in Centella asiatica hairy root culture by precursor feeding and elicitation
Centella asiatica contains various triterpenoids, including madecassoside, asiaticoside, madecassic acid, and asiatic acid. Among them, they are mainly contained in leaf, and among them asiaticoside is known as t...
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Open AccessCellulase recycling in high-solids enzymatic hydrolysis of pretreated empty fruit bunches
The lignocellulosic biomass feedstocks such as empty fruit bunches (EFBs) prove to be potential renewable resources owing to their abundance, low prices, and high carbohydrate contents. Generally, the conversi...
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Attributes of Polygonum multiflorum to transfigure red biotechnology
A vast array of plant-based compounds has enriched red biotechnology to serve the human health and food. A peculiar medicinal plant which was an element of traditional Chinese medicine for centuries as a liver...
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Enhanced production of phenolic compounds in hairy root cultures of Polygonum multiflorum and its metabolite discrimination using HPLC and FT-IR methods
Hairy root cultures (HRCs) are characterized by fast and unlimited root growth, and they have greater genetic stability than other cultivation methods. HRCs are known to accumulate phytochemical levels compara...
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Quality, safety and efficacy profiling of ginseng adventitious roots produced in vitro
Ginseng (Panax ginseng C. A. Meyer, Family Araliaceae) is one of the major medicinal and nutraceutical plants, which is native to oriental region. It is used worldwide as a popular herbal medicine because of its ...
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Biotic elicitation of ginsenoside metabolism of mutant adventitious root culture in Panax ginseng
Biotic elicitation is an important biotechnological strategy for triggering the accumulation of secondary metabolites in adventitious root cultures. These biotic elicitors can be obtained from safe, economical...
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Improvement of biosynthesis and accumulation of bioactive compounds by elicitation in adventitious root cultures of Polygonum multiflorum
We examined the effects of abiotic (methyl jasmonate [MeJA] and salicylic acid [SA]) and biotic (yeast extract and chitosan) elicitors for improvement of bioactive compounds production on adventitious root cul...
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Open AccessIn-vitro cytotoxicity assessment of carbon-nanodot-conjugated Fe-aminoclay (CD-FeAC) and its bio-imaging applications
We have investigated the cytotoxic assay of Fe-aminoclay (FeAC) nanoparticles (NPs) and simultaneous imaging in HeLa cells by photoluminescent carbon nanodots (CD) conjugation. Non-cytotoxic, photostable, and ...
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Hypericins: biotechnological production from cell and organ cultures
Hypericum perforatum L. (St. John’s wort), a perennial flowering plant native to Europe, is widely used as a medicinal plant and has a long history of its use in the treatment of various ailments...
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Biotechnological production of caffeic acid derivatives from cell and organ cultures of Echinacea species
Caffeic acid derivatives (CADs) are a group of bioactive compounds which are produced in Echinacea species especially Echinacea purpurea, Echinacea angustifolia, and Echinacea pallida. Echinacea is a popular herb...
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Ginsenosides: prospective for sustainable biotechnological production
Panax ginseng C.A. Meyer (ginseng) is a well-known medicinal plant that has been traditionally used in the oriental countries for centuries. Wild ginseng is a scarce and rare commodity. Field cul...
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The Binding of Hepatitis B Virus X Protein to Glioma-Associated Oncogene Homologue 1 and its Biological Characterization In vitro
Hepatitis B virus (HBV) X protein (HBx) is a 17-kDa transcriptional coactivator that plays a significant role in the regulation of genes involved in inflammation and cell survival. It has been known to be invo...
