21 Result(s)
within
Article
Seong Ho Kang
English
-
Article
Learning method of the ADALINE using the fuzzy logic system
We propose a learning method for the ADALINE. The proposed method exploits fuzzy logic system for automatic tuning of the weights of the ADALINE. The inputs of the fuzzy logic system are error and change of er...
-
Article
A Design of BASK System for Giga-bit Modem
We propose a binary amplitude shift keying (BASK) system of Giga-bit Modem without intermediate frequency process for the 60 GHz band. The proposed system consists of a high speed shutter of the transmitter an...
-
Article
Detection of single-molecule DNA hybridization by using dual-color total internal reflection fluorescence microscopy
We examined the use of prism-type simultaneous dual-color total internal reflection fluorescence microscopy (TIRFM) to probe DNA molecules at the single-molecule level. The system allowed the direct detection ...
-
Article
Open AccessPolarization of protective immunity induced by replication-incompetent adenovirus expressing glycoproteins of pseudorabies virus
Replication-incompetent adenoviruses expressing three major glycoproteins (gB, gC, and gD) of pseudorabies virus (PrV) were constructed and used to examine the ability of these glycoproteins to induce protecti...
-
Article
Evaluation of passive mixing behaviors in a pillar obstruction poly(dimethylsiloxane) microfluidic mixer using fluorescence microscopy
The rapid mixing of fluids passing through a microfluidic channel is very important for various applications of microfluidic systems. It has been a great challenge to achieve highly efficient mixing in a micro...
-
Article
Open AccessModulation of protective immunity against herpes simplex virus via mucosal genetic co-transfer of DNA vaccine with β2-adrenergic agonist
Cholera toxin, which has been frequently used as mucosal adjuvant, leads to an irreversible activation of adenylyl cyclase, thereby accumulating cAMP in target cells. Here, it was assumed that β2-adrenergic agoni...
-
Article
Quantification of UV-induced cyclobutane pyrimidine dimers using an oligonucleotide chip assay
A lesion-specific enzyme-induced DNA strand break assay was developed for an oligonucleotide chip for the determination of UVB-induced cyclobutane pyrimidine dimers (CPDs). A 20-mer of fluorophore-labeled and ...
-
Article
Concurrent p16 methylation pattern as an adverse prognostic factor in multiple myeloma: a methylation-specific polymerase chain reaction study using two different primer sets
Disruption of cell cycle control genes, including p16, is known to contribute to the cancerogenesis of multiple myeloma (MM). We investigated the methylation status of p16 and its association with common cytogene...
-
Article
Open AccessSuper-resolution of fluorescence-free plasmonic nanoparticles using enhanced dark-field illumination based on wavelength-modulation
Super-resolution imaging of fluorescence-free plasmonic nanoparticles (NPs) was achieved using enhanced dark-field (EDF) illumination based on wavelength-modulation. Indistinguishable adjacent EDF images of 10...
-
Article
Open AccessIn vitro generation of allo-reactive human NK cells from umblillical cord blood CD34+ progenitor cells
-
Article
Open AccessSilica-coated magnetic nanoparticles impair proteasome activity and increase the formation of cytoplasmic inclusion bodies in vitro
The potential toxicity of nanoparticles, particularly to neurons, is a major concern. In this study, we assessed the cytotoxicity of silica-coated magnetic nanoparticles containing rhodamine B isothiocyanate d...
-
Article
Open AccessAugmented 3D super-resolution of fluorescence-free nanoparticles using enhanced dark-field illumination based on wavelength-modulation and a least-cubic algorithm
Augmented three-dimensional (3D) subdiffraction-limited resolution of fluorescence-free single-nanoparticles was achieved with wavelength-dependent enhanced dark-field (EDF) illumination and a least-cubic algo...
-
Article
Open AccessQuality and freshness of human bone marrow-derived mesenchymal stem cells decrease over time after trypsinization and storage in phosphate-buffered saline
Human bone marrow-derived mesenchymal stem cells (hBM-MSCs) have been studied for their therapeutic potential. However, evaluating the quality of hBM-MSCs before transplantation remains a challenge. We address...
-
Article
3D super-localization of intracellular organelle contacts at live single cell by dual-wavelength synchronized fluorescence-free imaging
A fluorescence-free real-time three-dimensional (3D) super-localization method for the analysis of 3D structure of organelles (e.g., mitochondria-associated endoplasm reticulum [mito-ER] contacts) in live sing...
-
Article
Open AccessSilica-coated magnetic nanoparticles induce glucose metabolic dysfunction in vitro via the generation of reactive oxygen species
Nanoparticles are a useful material in biomedicine given their unique properties and biocompatibility; however, there is increasing concern regarding the potential toxicity of nanoparticles with respect to cel...
-
Article
BOK promotes erythropoiesis in a mouse model of myelodysplastic syndrome
Myelodysplastic syndromes are clonal hematopoietic stem cell disorders characterized by cytopenia and intramedullary apoptosis. BCL-2 Ovarian Killer (BOK) is a pro-apoptotic member of the BCL-2 family of prote...
-
Article
Open AccessDecrease in membrane fluidity and traction force induced by silica-coated magnetic nanoparticles
Nanoparticles are being increasingly used in biomedical applications owing to their unique physical and chemical properties and small size. However, their biophysical assessment and evaluation of side-effects ...
-
Article
Open AccessSilica-coated magnetic-nanoparticle-induced cytotoxicity is reduced in microglia by glutathione and citrate identified using integrated omics
Nanoparticles have been utilized in brain research and therapeutics, including imaging, diagnosis, and drug delivery, owing to their versatile properties compared to bulk materials. However, exposure to nanopa...
-
Article
Open AccessQuantifying intracellular trafficking of silica-coated magnetic nanoparticles in live single cells by site-specific direct stochastic optical reconstruction microscopy
Nanoparticles have been used for biomedical applications, including drug delivery, diagnosis, and imaging based on their unique properties derived from small size and large surface-to-volume ratio. However, co...
-
Article
Open AccessReal-time six-dimensional spatiotemporal tracking of single anisotropic nanoparticles in live cells by integrated multifunctional light-sheet nanoscopy
An integrated multifunctional light-sheet nanoscopy (iMLSN) combined with differential interference contrast, total internal reflection, epifluorescence, a super-resolution radial fluctuation-stream module, and a...
