13 Result(s)
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Article
Evaluation of zraP gene expression characteristics and construction of a lead (Pb) sensing and removal system in a recombinant Escherichia coli
A ZraP-based lead sensing and removal system was constructed in E. coli. It was regulated by the ZraS/ZraR two-component system. The expression profile of the zraP gene towards extracellular lead was studied via ...
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Article
Modification of the dynamic nature of the chimeric fumarate two-component system in Escherichia coli via positive feedback loop
A positive feedback loop was introduced to modify the dynamic behavior of fumarate sensing DcuSZ chimera TCS. To construct the positive feedback loop, the ompR gene was cloned downstream of the ompC promoter. The...
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Article
Engineering Escherichia coli to sense acidic amino acids by introduction of a chimeric two-component system
In an attempt to create an acidic amino acid-sensing Escherichia coli, a chimeric sensor kinase (SK)-based biosensor was constructed using Pseudomonas putida AauS. AauS is a sensor kinase that ultimately controls...
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Article
Construction of a high efficiency copper adsorption bacterial system via peptide display and its application on copper dye polluted wastewater
For the construction of an efficient copper waste treatment system, a cell surface display strategy was employed. The copper adsorption ability of recombinant bacterial strains displaying three different coppe...
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Article
Engineering a chimeric malate two-component system by introducing a positive feedback loop in Escherichia coli
Previous studies constructed a chimeric MalKZ two-component system to sense environmental malate. In this study, we used a positive feedback loop to accelerate and amplify the output signal indicating malate c...
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Article
Construction of methanol sensing Escherichia coli by the introduction of novel chimeric MxcQZ/OmpR two-component system from Methylobacterium organophilum XX
Methylobacterium organophilum XX is a type II facultative methylotroph that can grow on methanol. In M. organophilum XX, the MxcQ/MxcE two-component system (TCS) is involved in me...
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Article
Engineering chimeric two-component system into Escherichia coli from Paracoccus denitrificans to sense methanol
Escherichia coli does not have the methanol sensing apparatus, was engineered to sense methanol by employing chimeric two-component system (TCS) strategy. A chimeric FlhS/EnvZ (FlhSZ) chimeric histidine kinase (H...
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Article
Manganese and cobalt recovery by surface display of metal binding peptide on various loops of OmpC in Escherichia coli
In a cell-surface display (CSD) system, successful display of a protein or peptide is highly dependent on the anchoring motif and the position of the display in that anchoring motif. In this study, a recombina...
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Article
Development of bisphenol A-removing recombinant Escherichia coli by monomeric and dimeric surface display of bisphenol A-binding peptide
Peptide-displaying Escherichia coli cells were investigated for use in adsorptive removal of bisphenol A (BPA) both in Luria–Bertani medium including BPA or ATM thermal paper eluted wastewater. Two recombinant st...
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Article
Open AccessOn-farm colorimetric detection of Pasteurella multocida, Mannheimia haemolytica, and Histophilus somni in crude bovine nasal samples
This work modifies a loop-mediated isothermal amplification (LAMP) assay to detect the bovine respiratory disease (BRD) bacterial pathogens Pasteurella multocida, Mannheimia haemolytica, and Histophilus somni in ...
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Open AccessWearable adjunct ozone and antibiotic therapy system for treatment of Gram-negative dermal bacterial infection
The problematic combination of a rising prevalence of skin and soft tissue infections and the growing rate of life-threatening antibiotic resistant infections presents an urgent, unmet need for the healthcare ...
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Article
Adsorption of Lithium on Cell Surface as Nanoparticles through Lithium Binding Peptide Display in Recombinant Escherichia coli
Lithium is one of the highly used elements in various industries such as ceramics, polymer and battery. Considering the enormous demand of lithium based battery, the importance of lithium is getting higher in ...
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Article
Open AccessInstantly adhesive and ultra-elastic patches for dynamic organ and wound repair
Bioadhesive materials and patches are promising alternatives to surgical sutures and staples. However, many existing bioadhesives do not meet the functional requirements of current surgical procedures and inte...
