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Open AccessScreening for in planta protein-protein interactions combining bimolecular fluorescence complementation with flow cytometry
Understanding protein and gene function requires identifying interaction partners using biochemical, molecular or genetic tools. In plants, searching for novel protein-protein interactions is limited to protei...
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Article
Determination of the in vivo redox potential by one-wavelength spectro-microscopy of roGFP
For the quantitative analysis of molecular processes in living (plant) cells, such as the perception and processing of environmental and endogenous signals, new combinatorial approaches in optical and spectros...
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Article
K. Balasubramaniam and M. Burghard (Eds.): Carbon nanotubes. Methods and protocols
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Application of FLIM-FIDSAM for the in vivo analysis of hormone competence of different cell types
Background fluorescence derived from subcellular compartments is a major drawback in high-resolution live imaging, especially of plant cells. A novel technique for contrast enhancement of fluorescence images o...
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Article
Microcavities: tailoring the optical properties of single quantum emitters
We present a general review of different microresonator structures and how they can be used in future device applications in modern analytical methods by tailoring the optical properties of single quantum emit...
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Article
Open AccessRed fluorescence in reef fish: A novel signalling mechanism?
At depths below 10 m, reefs are dominated by blue-green light because seawater selectively absorbs the longer, 'red' wavelengths beyond 600 nm from the downwelling sunlight. Consequently, the visual pigments o...