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  1. Article

    Open Access

    Triacylglycerol synthesis by PDAT1 in the absence of DGAT1 activity is dependent on re-acylation of LPC by LPCAT2

    The Arabidopsis thaliana dgat1 mutant, AS11, has an oil content which is decreased by 30%, and a strongly increased ratio of 18:3/20:1, compared to wild type. Despite lacking a functional DGAT1, AS11 still manage...

    **gyu Xu, Anders S Carlsson, Tammy Francis, Meng Zhang… in BMC Plant Biology (2012)

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  2. No Access

    Article

    Abscisic acid metabolism and lipid accumulation of a cell suspension culture of Lesquerella fendleri

    Lesquerella fendleri (commonly known as “Fendler’s bladderpod” or “yellowtop”) is a member of the Brassicaceae and is an important seed oil-producing plant. The lipid profile of L. fendleri seed indicates potenti...

    Olesya A. Kharenko, L. Irina Zaharia… in Plant Cell, Tissue and Organ Culture (PCTO… (2011)

  3. No Access

    Article

    Increase in nervonic acid content in transformed yeast and transgenic plants by introduction of a Lunaria annua L. 3-ketoacyl-CoA synthase (KCS) gene

    Nervonic acid is a Very Long-Chain Monounsaturated Fatty Acid (VLCMFA), 24:1 Δ15 (cis-tetracos-15-enoic acid) found in the seed oils of Lunaria annua, borage, hemp, Acer (Purpleblow maple) and Tropaeolum speciosu...

    Yiming Guo, Elzbieta Mietkiewska, Tammy Francis, Vesna Katavic… in Plant Molecular Biology (2009)

  4. No Access

    Article

    Hairpin-RNA mediated silencing of endogenous FAD2 gene combined with heterologous expression of Crambe abyssinica FAE gene causes an increase in the level of erucic acid in transgenic Brassica carinata seeds

    The 3′-UTR of the FAD2 gene from Brassica carinata was cloned by PCR and used to prepare an intron-spliced hairpin RNA (ihpRNA) construct. Compared to that of the wild type (WT) background, this construct, when e...

    Elzbieta Mietkiewska, Travis L. Hoffman, Jennifer M. Brost… in Molecular Breeding (2008)

  5. No Access

    Article

    Production of 22:2Δ5,Δ13 and 20:1Δ5 in Brassica carinata and soybean breeding lines via introduction of Limnanthes genes

    Seed oils of meadowfoam (Limnanthes douglasii, L. alba) contain very long-chain fatty acids of strategic importance for a number of industrial applications. These include the monoene 20 1Δ5 and the diene 22:2Δ5,Δ...

    Ashok Jadhav, Elizabeth-France Marillia, Vivijan Babic… in Molecular Breeding (2005)

  6. No Access

    Article

    Molecular and biochemical characterization of an aminoalcoholphosphotransferase (AAPT1) from Brassica napus: effects of low temperature and abscisic acid treatments on AAPT expression in Arabidopsis plants and effects of over-expression of BnAAPT1 in transgenic Arabidopsis

    Aminoalcoholphosphotransferases (AAPT, EC 2.7.8.1 and EC 2.7.8.2) catalyze the transfer of CDP-aminoalcohols to sn-1, 2 diacylglycerol (DAG) to form phosphatidylaminoalcohols with the release of CMP. The Brassica...

    Qungang Qi, Yong-fen Huang, Adrian J. Cutler, Suzanne R. Abrams, David C. Taylor in Planta (2003)

  7. No Access

    Article

    Field testing of transgenic rapeseed cv. Hero transformed with a yeast sn-2 acyltransferase results in increased oil content, erucic acid content and seed yield

    A major goal of our research is to produce, by genetic manipulation, Brassica napus L. cultivars with higher levels of 22:1 in their seed oil than in present Canadian HEA cultivars developed through traditional b...

    David C. Taylor, Vesna Katavic, Jitao Zou, Samuel L. MacKenzie… in Molecular Breeding (2002)

  8. No Access

    Article

    Effects of antisense repression of an Arabidopsis thaliana pyruvate dehydrogenase kinase cDNA on plant development⋆

    Pyruvate dehydrogenase kinase (PDHK), a negative regulator of the mitochondrial pyruvate dehydrogenase (PDH) complex (mtPDC), plays a pivotal role in controlling mtPDC activity, and hence, the TCA cycle and ce...

    Jitao Zou, Qungang Qi, Vesna Katavic, Elizabeth-F. Marillia… in Plant Molecular Biology (1999)

  9. No Access

    Article

    Cloning of a cDNA encoding the 21.2 kDa oleosin isoform from Arabidopsis thaliana and a study of its expression in a mutant defective in diacylglycerol acyltransferase activity

    A full-length cDNA clone (pA23) of 832 bp encoding an oleosin from Arabidopsis thaliana was isolated by differential screening of a silique-specific cDNA library with probes prepared from poly(A)+ RNA isolated fr...

    Jitao Zou, Stephen J. Brokx, David C. Taylor in Plant Molecular Biology (1996)

  10. No Access

    Article

    Storage-protein regulation and lipid accumulation in microspore embryos of Brassica napus L.

    Embryos derived in vitro from isolated microspores of Brassica napus L. were compared with their zygotic counterparts. Parameters investigated included storage-protein accumulation and gene expression, fattyacid ...

    David C. Taylor, Nikolaus Weber, Edward W. Underhill, M. Keith Pomeroy… in Planta (1990)