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Article
Identification, duplication, evolution and expression analyses of caleosins in Brassica plants and Arabidopsis subspecies
Caleosins are a class of Ca2+ binding proteins that appear to be ubiquitous in plants. Some of the main proteins embedded in the lipid monolayer of lipid droplets, caleosins, play critical roles in the degradatio...
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Article
Abscisic acid metabolism and lipid accumulation of a cell suspension culture of Lesquerella fendleri
Lesquerella fendleri (commonly known as “Fendler’s bladderpod” or “yellowtop”) is a member of the Brassicaceae and is an important seed oil-producing plant. The lipid profile of L. fendleri seed indicates potenti...
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Article
Open AccessTransgenic increases in seed oil content are associated with the differential expression of novel Brassica-specific transcripts
Seed oil accumulates primarily as triacylglycerol (TAG). While the biochemical pathway for TAG biosynthesis is known, its regulation remains unclear. Previous research identified microsomal diacylglycerol acyl...
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Article
Hairpin-RNA mediated silencing of endogenous FAD2 gene combined with heterologous expression of Crambe abyssinica FAE gene causes an increase in the level of erucic acid in transgenic Brassica carinata seeds
The 3′-UTR of the FAD2 gene from Brassica carinata was cloned by PCR and used to prepare an intron-spliced hairpin RNA (ihpRNA) construct. Compared to that of the wild type (WT) background, this construct, when e...
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Article
Production of 22:2Δ5,Δ13 and 20:1Δ5 in Brassica carinata and soybean breeding lines via introduction of Limnanthes genes
Seed oils of meadowfoam (Limnanthes douglasii, L. alba) contain very long-chain fatty acids of strategic importance for a number of industrial applications. These include the monoene 20 1Δ5 and the diene 22:2Δ5,Δ...
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Article
Field testing of transgenic rapeseed cv. Hero transformed with a yeast sn-2 acyltransferase results in increased oil content, erucic acid content and seed yield
A major goal of our research is to produce, by genetic manipulation, Brassica napus L. cultivars with higher levels of 22:1 in their seed oil than in present Canadian HEA cultivars developed through traditional b...