14 Result(s)
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Article
Dasheng Zhang
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Article
Large Stokes shift fluorescent RNAs for dual-emission fluorescence and bioluminescence imaging in live cells
Fluorescent RNAs, aptamers that bind and activate small fluorogenic dyes, have provided a particularly attractive approach to visualizing RNAs in live cells. However, the simultaneous imaging of multiple RNAs ...
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Open AccessDesign of a palette of SNAP-tag mimics of fluorescent proteins and their use as cell reporters
Naturally occurring fluorescent proteins (FPs) are the most widely used tools for tracking cellular proteins and sensing cellular events. Here, we chemically evolved the self-labeling SNAP-tag into a palette o...
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Improving the spatial resolution of GRACE-based groundwater storage estimates using a machine learning algorithm and hydrological model
The low-resolution characteristic of Gravity Recovery and Climate Experiment (GRACE) satellite data greatly limits their application in many fields at regional or local scales. Aiming to overcome this limitati...
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Spatial and temporal characteristics of annual and seasonal precipitation variation in Shijiazhuang region, north China
Understanding spatial and temporal characteristics of annual and seasonal precipitation variation is vital in watershed development and management. In this study, we investigated the spatial and temporal varia...
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Open AccessThe construction of recombinant Lactobacillus casei vaccine of PEDV and its immune responses in mice
Porcine epidemic diarrhea (PED) is a contagious intestinal disease caused by porcine epidemic diarrhea virus (PEDV) characterized by vomiting, diarrhea, anorexia, and dehydration, which have caused huge econom...
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The synergetic effect of antimony (Sb2O3) and melamine cyanurate (MCA) on the flame-retardant behavior of silicon rubber
The silicone rubber composites with antimony trioxide (Sb2O3) and melamine cyanurate (MCA) additive were fabricated. The results of scanning electron microscope are shown that the Sb2O3 and MCA are uniformly disp...
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Numerical Simulation of Multi-Water-Source Artificial Recharge of Aquifer: A Case Study of the Mi-Huai-Shun Groundwater Reservoir
Artificial recharge is an effective way to alleviate water shortages and excessive groundwater exploitation. In this study, to explore environmental changes of surface water and groundwater during multi-water-...
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Visualizing RNA dynamics in live cells with bright and stable fluorescent RNAs
Fluorescent RNAs (FRs), aptamers that bind and activate fluorescent dyes, have been used to image abundant cellular RNA species. However, limitations such as low brightness and limited availability of dye/apta...
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Micropropagation of Nelumbo nucifera ‘Weishan Hong’ through germfree mature embryos
Asian lotus (Nelumbo nucifera Gaertn.) is an economically important aquatic plant that is widely cultivated in Asia. It is mainly propagated by dividing rhizomes, which maintains genotype stability but is also la...
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Transcriptome exploration for further understanding of the tropane alkaloids biosynthesis in Anisodus acutangulus
Tropane alkaloids (TAs) such as anisodamine, anisodine, hyoscyamine and scopolamine are extensively used in clinical practice as anticholinergic agents. Anisodus acutangulus produces TAs in root tissue, and altho...
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Open AccessCo-overexpression of geraniol-10-hydroxylase and strictosidine synthase improves anti-cancer drug camptothecin accumulation in Ophiorrhiza pumila
Camptothecin (CPT) belongs to a group of monoterpenoidindole alkaloids (TIAs) and its derivatives such as irinothecan and topothecan have been widely used worldwide for the treatment of cancer, giving rise to ...
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SNX13 reduction mediates heart failure through degradative sorting of apoptosis repressor with caspase recruitment domain
Heart failure (HF) is associated with complicated molecular remodelling within cardiomyocytes; however, the mechanisms underlying this process remain unclear. Here we show that sorting nexin-13 (SNX13), a memb...
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Tom70 serves as a molecular switch to determine pathological cardiac hypertrophy
Pathological cardiac hypertrophy is an inevitable forerunner of heart failure. Regardless of the etiology of cardiac hypertrophy, cardiomyocyte mitochondrial alterations are always observed in this context. Th...
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Open AccessAn effective approach for identification of in vivo protein-DNA binding sites from paired-end ChIP-Seq data
ChIP-Seq, which combines chromatin immunoprecipitation (ChIP) with high-throughput massively parallel sequencing, is increasingly being used for identification of protein-DNA interactions in vivo in the genome. H...
