12,666 Result(s)
within
English
Life Sciences
Organic Chemistry
**yi **a
Article
-
Article
A single-bead decode strategy using electrospray ionization mass spectrometry and a new photolabile linker: 3-Amino-3-(2-nitrophenyl)propionic acid
A new linker that employs a photosensitive 3-ammo-3-(2-nitrophenyl)propionyl functionality (ANP-resin) has been developed for the preparation of C-terminal carboxamides. A wide range of carboxamides were prepa...
-
Article
Map** the detailed specificity of a calcium-dependent monoclonal antibody through the use of soluble positional scanning combinatorial libraries: Identification of potent calcium-independent antigens
The detailed specificity of monoclonal antibody M1, which has been reported to bind in a calcium-dependent manner to the ‘FLAG’ sequence DYKDDDDK-NH2, was examined using soluble hexa- and decapeptide positional s...
-
Article
In vitro selection methodologies to probe RNA function and structure
In vitro selection, or SELEX, has been used both to characterize the interaction of natural nucleic acids with proteins and to generate novel nucleic acid-binding species, or aptamers. Although numerous report...
-
Article
A combinatorial method for constructing libraries of long peptides displayed by filamentous phage
We describe the construction and screening of a random peptide library displayed by filamentous phage. The peptides are expressed in multiple copies on the filamentous phage M13 as amino-terminal fusions with ...
-
Article
Molecular diversity comes of age !
-
Article
Multiple simultaneous synthesis of phenolic libraries
A series of analogous arrays of small, non-peptidyl, non-oligomeric compounds were synthesized on polystyrene resin. With the aid of a functionally differentiated phenolic scaffold, the batch preparation of un...
-
Article
Libraries of random-sequence polypeptides produced with high yield as carboxy-terminal fusions with ubiquitin
Libraries of random-sequence polypeptides have been shown to be valuable sources of novel molecules possessing a variety of useful biologic-like activities, some of which may hold promise as potential vaccines...
-
Article
Adaptive walks with noisy fitness measurements
Adaptive walks constitute an optimization technique for searching a space of possible solutions, for example, a space of different molecules. The goal is to find a point in space (a molecule) that is optimal o...
-
Article
Structural aspects of antibody-antigen interaction revealed through small random peptide libraries
Two small random peptide libraries, one composed of 4550 dodecapeptides and one of 8000 tripeptides, were synthesized in newly developed credit-card format miniPEPSCAN cards (miniPEPSCAN libraries). Each pepti...
-
Article
A new combination of protecting groups and links for encoded synthetic libraries suited for consecutive tests on the solid phase and in solution
A strategy for high-throughput evaluation of combinatorial compound libraries is reported, which circumvents the necessity to test complex mixtures. The method is based on a new combination of protecting group...
-
Article
Antibiotic activity of polyketide products derived from combinatorial biosynthesis: Implications for directed evolution
A library of over 100 polyketides, generated via combinatorial cloning of genes encoding subunits of aromatic polyketide synthases, was screened for molecules capable of inhibiting the growth of grampositive b...
-
Article
Evolutionary molecular engineering in Japan
-
Article
Utilization of multiple phage display libraries for the identification of dissimilar peptide motifs that bind to a B7-1 monoclonal antibody
Seven random peptide libraries (two displaying linear peptides and five displaying cysteine-constrained peptides) were constructed as gene III fusion proteins of the bacteriophage fd-tet. These libraries were ...
-
Article
Directed evolution studies with combinatorial libraries of T4 lysozyme mutants
Gene duplication with divergence to new functions has been an important mechanism in protein evolution. However, the questions of how many new functions can arise from a particular ancestral gene and how many ...
-
Article
Combinatorial synthesis of small-molecule libraries using 3-amino-5-hydroxybenzoic acid
A non-peptide library of 2001 compounds has been prepared utilizing solid-phase techniques. The split/combine method was demonstrated to work well to form mixtures of compounds based on 3-amino-5-hydroxybenzoi...
-
Article
High-throughput screening strategies to identify inhibitors of protein-protein interactions
-
Article
Solid phase organic synthesis (SPOS): A novel route to diketopiperazines and diketomorpholines
The solid phase synthesis of libraries containing a 1,3,4,6-tetrasubstituted-2,5-diketo-1,4-piperazine scaffold (DKP) or a 3,4,6-trisubstituted-2,5-diketo-1,4-morpholme scaffold (DKM) from α-bromocarboxylic ac...
-
Article
Synthetic library techniques: Subjective (biased and generic) thoughts and views
Various aspects of synthetic diversity generation and screening are discussed. Controversial issues are raised and different points of view are presented. We hope the article will stimulate thinking about the ...
-
Article
Structurally homogeneous and heterogeneous synthetic combinatorial libraries
We have designed and synthesized structurally homogeneous and heterogeneous nonpeptide libraries. Structurally homogeneous libraries are characterized by the presence of one common structural unit, a scaffold,...
-
Article
Bifunctional scaffolds as templates for synthetic combinatorial libraries
A small-molecule synthetic combinatorial library was designed and synthesized that features potential pharmacophores attached to a variety of small cyclic scaffolds. The synthesis of the library involved rando...
