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Optical Estimation of Bioelectric Patterns in Living Embryos
Fluorescent lifetime imaging (FLIM) is a powerful tool for visualizing physiological parameters in vivo. We present here a 3-dye strategy for map**...
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Identifying Key In Silico Knockout for Enhancement of Limonene Yield Through Dynamic Metabolic Modelling
Living cells display dynamic and complex behaviors. To understand their response and to infer novel insights not possible with traditional...
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Purified Versus Plasma Alpha-1 Antitrypsin Effects on Cellular Activities
By adding purified exogenous alpha-1 antitrypsin (AAT) to peripheral blood mononuclear cells in 20% autologous serum and measuring AAT-induced...
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Silencing Very-Low-Density Lipoprotein Receptor Reveals Alpha-1 Antitrypsin Role in HIV Infectivity
Here we describe methods for investigating alpha-1 antitrypsin (AAT) and very-low-density lipoprotein receptor (VLDLR) interactions with infectious...
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Amniotic Membrane and Amniotic Epithelial Cell Culture
Amniotic membrane (AM) is considered an important medical device for applications in regenerative medicine. The therapeutic properties of AM are due...
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Array-Based Comparative Genomic Hybridization for the Detection of Copy Number Alterations in Single Cells
Comprehensive genome-wide analyses of single cells represent an important tool for clinical applications, such as pre-implantation diagnostic and...
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3D Breast Cancer Model on Silk Fibroin–Integrated Microfluidic Chips
To imitate in vivo environment of cells, microfluidics offer controllable fashions at micro-scale and enable regulate flow-related parameters...
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Preparation of Corneal Tissue Matrix Bioink for 3D Bioprinting
There are many protocols available to decellularize tissues for the preparation of bioink for 3D bioprinting purposes. Almost all the methods...
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Generation of iPSC Cell Lines from Patients with Sex Chromosome Aneuploidies
Somatic cell reprogramming allows the generation of human induced pluripotent stem cells (iPSCs) from patient’s cells. The derived iPSCs provide an...
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Induction of Meiotic Initiation in Long-Term Mouse Spermatogonial Stem Cells Under Retinoid Acid and Nutrient Restriction Conditions
Spermatogonial stem cells (SSCs) produce haploid sperm via mitosis and meiosis in vivo. Although the technique to culture mouse SSCs has been well...
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Purification by STA-PUT Technique of Male Germ Cells from Single Mouse and RNA-Extraction for Transcriptomic Analysis
Transcriptomic analyses of germ cells at different stages of differentiation have shed light on the transcriptional and post-transcriptional...
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In Vitro Culture of White Adipose Tissue
White adipose tissue (WAT) plays a crucial endocrine organ that regulates blood glucose and lipid levels, satiety, and inflammation. Before the...
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Quality Control Assessment of Human Adipose-Derived Hydrogels
Decellularized human-adipose tissue (hDAT) can serve as an alternative to two-dimensional monolayer culture and current ECM hydrogels due to its...
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Feline Adult Adipose Tissue-Derived Multipotent Stromal Cell Isolation and Differentiation
Cats are among the most popular household pets. However, compared to other species, there is little information specific to feline adult mesenchymal...
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Isolation and Characterization of Testis Macrophages Using Flow Cytometry
Testis-resident macrophages are first responders of the innate immune system against pathogens. They also exert day-to-day functions that are poorly...
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Three-Dimensional Imaging of Macrophages in Complete Organs
The introduction of the light-sheet microscope has facilitated the analysis of complete tissues for the presence of all cells and their location in...
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Elucidating Immune Monitoring of Tissue-Resident Macrophages by Intravital Microscopy
Intravital microscopy is an invaluable tool to study in real time the dynamic behavior of leukocytes in vivo. We describe herein a simple protocol...
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Fate-Map** of Hematopoietic Stem Cell-Derived Macrophages
Macrophages are cells of the innate immune system, which contribute to the maintenance of tissue homeostasis and form the first line of defense...
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Cellular Blood Flow Modeling with HemoCell
Many of the intriguing properties of blood originate from its cellular nature. Bulk effects, such as viscosity, depend on the local shear rates and...
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Alchemical Free Energy Workflows for the Computation of Protein-Ligand Binding Affinities
Alchemical free energy methods can be used for the efficient computation of relative binding free energies during preclinical drug discovery stages....