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GRAMM Web Server for Protein Docking
Prediction of the structure of protein complexes by docking methods is a well-established research field. The intermolecular energy landscapes in...
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Molecular Dynamics Simulation-Based Prediction of Glycosaminoglycan Interactions with Drug Molecules
Glycosaminoglycans (GAGs) are a class of long linear anionic periodic polysaccharides. Their biological activities are very broad including tissue...
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Detection of Beta-Glucan Contamination in Nanoparticle Formulations
Beta-glucans with diverse chemical structures are produced by a variety of microorganisms and are commonly found in microbial cell walls....
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Advancements in Nanoparticle Characterization
Nanotechnology for drug delivery has made significant advancements over the last two decades. Innovations have been made in cancer research and...
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Detection of Nanoparticle-Mediated Total Oxidative Stress in T Cells Using CM-H2DCFDA Dye
Oxidative stress is commonly observed in cells following exposure to nanoparticles. Both negative (e.g., cytotoxicity and inflammation) and...
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Analysis of Nanoparticles’ Effects on Drug-Induced Psoriasis
Psoriasis, an auto-inflammatory disorder, has major manifestations in the skin but can affect other organs. Currently, this condition has no cure,...
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Detection of Nanoparticle-Mediated Change in Mitochondrial Membrane Potential in T Cells Using JC-1 Dye
Alterations in mitochondrial membrane potential are associated with the generation of reactive oxygen species and cell death. While eliminating...
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Analysis of Nanoparticles’ Potential to Induce Autoimmunity
Autoimmune responses are characterized by the presence of antibodies and lymphocytes specific to self or so-called autoantigens. Among such...
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Antigen-Specific Stimulation of CD8+ T Cells by Murine Bone Marrow–Derived Dendritic Cells After Treatment with Nanoparticles
The assessment of antigen presentation by dendritic cells and subsequent antigen-dependent activation of T lymphocytes is a critical step underlying...
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In Vitro and In Vivo Methods for Analysis of Nanoparticles' Potential to Induce Delayed-Type Hypersensitivity Reactions
Delayed-type hypersensitivity (DTH) reactions are among the common reasons for drug withdrawal from clinical use during the post-marketing stage....
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Immunophenoty**, Part II: Analysis of Nanoparticle Effects on the Composition and Activation Status of Human Peripheral Blood Mononuclear Cells
The use of nanoparticles as drug delivery carriers requires analysis of their safety, which among other tests, includes immunotoxicity. Nanoparticles...
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Assessment of Protein Binding Using Asymmetric-Flow Field-Flow Fractionation Combined with Multi-angle Light Scattering and Dynamic Light Scattering
Asymmetric-flow field-flow fractionation (AF4) is a valuable tool to separate and assess different size populations in nanotherapeutics. When coupled...
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Nanoparticle Size Distribution and Stability Assessment Using Asymmetric-Flow Field-Flow Fractionation
Nanomaterials are inherently polydisperse. Traditional techniques, such as the widely used batch-mode dynamic light-scattering (DLS) analysis, are...
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Analysis of Nanoparticle Adjuvant Properties
Nanoparticles can be engineered for targeted antigen delivery to immune cells and for stimulating an immune response to improve the antigen...
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Evaluation of Nanomedicine Tissue Distribution and Stability by Alexa Fluor 488 and PEG Immunohistochemistry
A primary issue with nanomedicine biological evaluation is determination of nanoparticle carrier tissue distribution and stability. Here we present a...
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Current Considerations and Practical Solutions for Overcoming Nanoparticle Interference with LAL Assays and Minimizing Endotoxin Contamination
Monitoring endotoxin contamination in drugs and medical devices is required to avoid pyrogenic responses and septic shock in patients receiving these...
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Quantitation of Active Pharmaceutical Ingredients in Polymeric Drug Products Using Elemental Analysis
Polymeric prodrugs have gained significant popularity as a strategy to enhance the bioavailability and improve the pharmacokinetic properties of...
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A Static Headspace Gas Chromatography Method for Quantitation of Residual Solvents in Nanoformulations
Various organic solvents are widely used in the manufacturing, processing, and purification of drug substances, drug products, formulations,...
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Assessment of Temperature-Dependent Drug Release of Solubilizing Nanoformulations Using the SITUA
The evaluation of temperature-dependent drug release for solubilizing nanoformulations uses a modification of the stable isotope ultrafiltration...
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Particle Size and Concentration Measurement Using the Spectradyne nCS1 Instrument
This protocol describes the use of the Spectradyne nCS1 instrument to measure the particles per mL concentration and size of nanoparticles. The...