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Article
Open AccessA software pipeline for processing and identification of fungal ITS sequences
Fungi from environmental samples are typically identified to species level through DNA sequencing of the nuclear ribosomal internal transcribed spacer (ITS) region for use in BLAST-based similarity searches in th...
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Article
Open AccessMining metadata from unidentified ITS sequences in GenBank: A case study in Inocybe (Basidiomycota)
The lack of reference sequences from well-identified mycorrhizal fungi often poses a challenge to the inference of taxonomic affiliation of sequences from environmental samples, and many environmental sequence...
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Article
Open AccessApproaching the taxonomic affiliation of unidentified sequences in public databases – an example from the mycorrhizal fungi
During the last few years, DNA sequence analysis has become one of the primary means of taxonomic identification of species, particularly so for species that are minute or otherwise lack distinct, readily obta...
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Article
Open AccessgalaxieEST: addressing EST identity through automated phylogenetic analysis
Research involving expressed sequence tags (ESTs) is intricately coupled to the existence of large, well-annotated sequence repositories. Comparatively complete and satisfactory annotated public sequence libra...