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Article
Transgenic tobacco expressing Vitreoscilla hemoglobin exhibits enhanced growth and altered metabolite production
The gene for Vitreoscilla hemoglobin (VHb) has been introduced and expressed in Nicotiana tabaccum (tobacco). Transgenic tobacco plants expressing VHb exhibited enhanced growth, on average 80–100% more dry weight...
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Article
An artificial bifunctional enzyme, γ-glutamyl kinase/γ-glutamyl phosphate reductase, improves NaCl tolerance when expressed in Escherichia coli
An artificial bifunctional enzyme, γ-glutamyl kinase/γ-glutamyl phosphate reductase, was obtained by fusing the Escherichia coli genes proA and proB. The proB gene was fused to the 5′-end of the proA gene with a ...
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Article
Enhanced NaCl Stress Tolerance in Transgenic Tobacco Expressing Bacterial Choline Dehydrogenase
The intracellular accumulation of osmoprotectants, such as glycine betaine and other low molecular weight compounds, is a well investigated response of environmental stress occurring in a wide range of organis...
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Article
Structure of a cyclic peptide with a catalytic triad, determined by computer simulation and NMR spectroscopy
We report the design of a cyclic, eight-residue peptide that possesses the catalytic triad residues of the serine proteases. A manually built model has been relaxed by 0.3 ns of molecular dynamics simulation a...
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Article
Engineering of lactose metabolism inE. coli by introducing β-galactosidase/galactokinase fusion enzymes
A series of plasmids encoding β-galactosidase/galactokinase fusion proteins with connecting linkers of different lengths and properties separating the enzyme moieties were made.E. coli cells harbouring the genes ...
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Article
Continuous Regeneration of NAD(H) Covalently Bound to a Cysteine Genetically Engineered into Glucose Dehydrogenase
We introduced a cysteine residue on the surface of glucose dehydrogenase from Bacillus subtilis using site-directed muta-genesis. To this mutant, an NAD-analogue was covalently attached by a disulphide bridge so ...
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Article
Preparation of a Soluble Bifunctional Enzyme by Gene Fusion
Within the cellular framework are a number of sequentially operating enzyme systems, in many cases in the form of bi- or even multifunctional proteins each consisting of a single type of polypeptide chain but ...
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Article
Formation of proinsulin by immobilized Bacillus subtilis
There has been an increasing interest in the use of immobilized cells for the production of pharmaceuticals as well as for products such as high fructose syrup or ethanol1. Some of these compounds are now produce...
