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Article
Lectinochemical studies on the glyco-recognition factors of a Tn (GalNAcα1→Ser/Thr) specific lectin isolated from the seeds of Salvia sclarea
The lectin extracted from the seeds of Salvia sclarea (SSL) recognizes the Tn antigen (GalNAc α1→Ser/Thr) expressed in certain human carcinomas. In previous studies, knowledge of the binding properties of SSL was...
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Article
Structural basis for oligosaccharide-mediated adhesion of Pseudomonas aeruginosa in the lungs of cystic fibrosis patients
Pseudomonas aeruginosa galactose- and fucose-binding lectins (PA-IL and PA-IIL) contribute to the virulence of this pathogenic bacterium, which is a major cause of morbidity and mortality in cystic fibrosis patie...
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Article
Carbohydrate Structural Units in Glycosphingolipids as Receptors for Gal and GalNAc Reactive Lectins
Glycosphingolipids (GSLs) contain many carbohydrate epitopes or crypto-glycotopes for Gal and GalNAc reactive lectins. Many of them are in the nervous system and function as important receptors in various life...
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Book
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Chapter
Lectin and Anti-Carbohydrate Antibody Assays Using Chemically Modified Ligands
Microtiter plate assays and ‘lectinoblotting’ with the use of biotinylated lectins are tive and easy to perform methods that can be combined with simple procedures of mical modifications of glycoproteins immob...
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Chapter
Expression of Binding Properties of Gal/GalNAc Reactive Lectins by Mammalian Glycotopes
Expression of the binding properties of Gal/GalNAc specific lectins, based on the affinity of decreasing order of mammalian glycotopes (determinants) rather than saccharide inhibition pattern, is probably one ...
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Chapter
A Guide to the Carbohydrate Specificities of Applied Lectins-2
The lectins, that can be used as tools to study glycobiological systems are defined as applied lectins (1–14).They are easily obtained, stable and have their own binding specificity extending beyond the monosacch...
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Chapter
Binding Properties and Applications of Aplysia Gonad Lectin
Adult Aplysia gonad contains high levels of a galactophilic lectin (MW around 65 kDa; composed of 2 subunits of apparent single species). It binds galactose and various a/ß-galactosides (but not N-acetylgalactosa...
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Article
Book review
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Chapter
Preparation of Biotinylated Lectins and Application in Microtiter Plate Assays and Western Blotting
The biotinylation of lectins via amino groups with biotinamidocaproate N-hydroxysuccinimide ester provided reagents with fully preserved carbohydrate-binding activity which were detectable with high sensitivity u...
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Article
Vicia villosa B4 lectin is the second anti-Tn lectin shown to react better with blood group N than M antigen
Earlier studies showed thatMoluccella laevis lectin, which has anti-Tn specificity, reacts more strongly with native or desialylated blood group N glycophorin A than with the respective glycophorins of blood grou...
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Article
Structure, biosynthesis, and function of salivary mucins
The glandular secretions of the oral cavity lining the underlying buccal mucosa are highly specialized fluids which provide lubrication, prevent mechanical damage, protect efficiently against viral and bacteri...
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Book
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Chapter
Immunochemical Analysis of Lipopolysaccharides with 2-D Gel Electrophoresis and Monoclonal Antibodies
Phenol extracted, alkali treated lipopolysaccharide from vaccine strain (S19) Brucella abortus was demonstrated by twodimensional gel electrophoresis to have at least ten silvers taining analogues. When tested...
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Chapter
A Guide for Carbohydrate Specificities of Lectins
Lectins are carbohydrate-binding proteins of nonimmune origin, which have been widely used in the fields of cell biology, biochemistry and histochemistry to isolate and/or to characterize cell surface carbohyd...
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Chapter
Structural Concepts of the Human Blood Group A, B, H, Lea, Leb, I and i Active Glycoproteins Purified from Human Ovarian Cyst Fluid
The chemical structures of the blood group A, B, H, Lea, Leb, I and i determinants in water soluble blood group substances isolated from human ovarian cyst fluid have been studied for over four decades and are we...
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Chapter
Biochemistry and Lectin Binding Properties of Mammalian Salivary Mucous Glycoproteins
Mucus is a complex exocrine secretion that covers the epithelial linings of higher animals. This secretion is derived from different types of epithelial glands which are composed of a variety of specialized ce...
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Chapter
Structural and Immunochemical Aspects of Brucella Abortus Endotoxins
The smooth lipopolysaccharide (sLPS)-protein complex of Brucella abortus has been identified as the immuno-dominant component, which reacts with antibody in the serological tests generally used for diagnosis of b...
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Chapter
Differential Binding Properties of GalNAc and/or Gal Specific Lectins
The binding properties of lectins have been used to study the structural and functional role of cell surface carbohydrates (1–6), to detect sugar moieties on normal and neoplastic cell surfaces (1), to isolate...
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Article
Differential binding characteristics and applications of dGalβ1→3dGalNAc specific lectins
The binding properties of Arachis hypogaea (PNA), Bauhinia vurpurea alba (BPL), Maclura pomifera (MPL) and Sophora japonica (SJL) lectins were studied by quantitative precipitin and precipitin inhibition assays, ...
