24 Result(s)
within
Protocol
Li**g Du
Biomedicine
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Protocol
Monitoring I-SceI-Induced Double-Strand Break Repair in DT40 Cells
The chicken B lymphocyte cell line DT40 is now a well-established system for studying DNA repair in vertebrates. Central to its utility is its ready genetic tractability allowing for the creation of isogenic m...
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Protocol
ENU Mutagenesis in Mice
Forward genetics has led to many “breakthrough” discoveries, and with the mouse genome almost fully sequenced, the creation of phenotypes through random germline mutagenesis has become an efficient means by wh...
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Protocol
Protein–Protein Interactions
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Protocol
Isolation of Human Skin Dendritic Cells by In Vitro Migration
In the human skin, various types of antigen-presenting cells (APC) are present. In the epidermis, they are identified ultrastructurally as Langerhans cells (LC) by the presence of Birbeck granules. LC are cons...
