58 Result(s)
within
English
Biotechnology
Da** Chen
Protocol
-
Protocol
Micromachined Optical Fiber Sensors for Biomedical Applications
Optical fibers revolutionized the rate of information reception and transmission in telecommunications. The revolution has now extended to the field of physicochemical sensing. Optical fiber sensors (OFSs) hav...
-
Protocol
Electrochemistry Coupling Localized Surface Plasmon Resonance for Biochemical Detection
Localized surface plasmon resonance (LSPR) associated with metal nanostructures has developed into highly useful sensor techniques. LSPR spectroscopy often shows absorption peaks which could be used for biomed...
-
Protocol
Antibody Aggregate Removal Using a Mixed-Mode Chromatography Resin
In monoclonal antibody (mAb) production, aggregates represent a major class of product-related impurities that needs to be removed by the downstream process. Protein A chromatography is generally less effectiv...
-
Protocol
Simulation of pH-Dependent, Loop-Based Membrane Protein Gating Using Pretzel
Bacterial porins often exhibit ion conductance and gating behavior which can be modulated by pH. However, the underlying control mechanism of gating is often complex, and direct inspection of the protein struc...
-
Protocol
Protein Transport Studied by a Model Asymmetric Membrane Army Arranged in a Dimple Chip
Reconstituted model membrane systems are powerful platforms to tackle interesting problems existing in membrane biology. One of the barriers to efficient drug delivery, as therapeutics to disease, is the physi...
-
Protocol
Vitrification of Heart Valve Tissues
Application of the original vitrification protocol used for pieces of heart valves to intact heart valves has evolved over time. Ice-free cryopreservation by Protocol 1 using VS55 is limited to small samples (...
-
Protocol
Vaccine Delivery with a Detoxified Bacterial Toxin
It is still a challenge to develop needle-free mucosal vaccines. Despite progress in the development of the influenza vaccine, it must be reformulated annually because of antigenic changes in circulating influ...
-
Protocol
Bioprinting of Complex Vascularized Tissues
Functional vasculature is crucial for the maintenance of living tissues via the transport of oxygen, nutrients, and metabolic waste products. As a result, insufficient vascularization in thick engineered tissu...
-
Protocol
Designing and Constructing Artificial Small RNAs for Gene Regulation and Carbon Flux Redirection in Photosynthetic Cyanobacteria
Photosynthetic cyanobacteria are not only model organisms for studying photosynthesis and biological cycling of carbon in biosphere but also potential “green microbial factories” to produce renewable fuels and...
-
Protocol
Protein Analyte Sensing with an Outer Membrane Protein G (OmpG) Nanopore
Nanopore sensing is a powerful lab-on-a-chip technique that allows for the analysis of biomarkers present in small sample sizes. In general, nanopore clogging and low detection accuracy arise when the sample b...
-
Protocol
A Selective Activity-Based Approach for Analysis of Enzymes with an OmpG Nanopore
Many enzymatic activity assays are based on either (1) identifying and quantifying the enzyme with methods such as western blot or enzyme-linked substrate assay (ELISA) or (2) quantifying the enzymatic reactio...
-
Protocol
CdS Quantum Dots Modified Photoelectrochemical Biosensor for TATA-Binding Protein Probing
The photoelectrochemical (PEC) biosensor, in which light is utilized to excite the photoactive species and current is employed as the detection signal, is a newly appeared yet dynamically develo** technique ...
-
Protocol
Detection of Tetanus Antibody Applying a Cu-Zn-In-S/ZnS Quantum Dot-Based Lateral Flow Immunoassay
Lateral flow test strip (LFTS) enables rapid, portable, and low-cost point-of-care testing (POCT) diagnosis. Quantum dots (QDs), which are fluorescent semiconductor nanocrystals with distinctive and unique pho...
-
Protocol
Generation of Rabbit Models by Gene Editing Nucleases
Due to the lack of germline transmitting pluripotent stem cells (PSCs) cell lines and the extreme difficulty of somatic cell nuclear transfer (SCNT) in rabbit, the gene targeting technology in rabbit was laggi...
-
Protocol
A Novel Colorimetric PCR-Based Biosensor for Detection and Quantification of Hepatitis B Virus
Hepatitis B virus (HBV) can cause viral infection that attacks the liver and it is a major global health problem that puts people at a high risk of death from cirrhosis of the liver and liver cancer. HBV has i...
-
Protocol
Microfluidic “Pouch” Chips for Immunoassays and Nucleic Acid Amplification Tests
Microfluidic cassettes (“chips”) for processing and analysis of clinical specimens and other sample types facilitate point-of-care (POC) immunoassays and nucleic acid based amplification tests. These single-us...
-
Protocol
qFlow Cytometry-Based Receptoromic Screening: A High-Throughput Quantification Approach Informing Biomarker Selection and Nanosensor Development
Nanosensor-based detection of biomarkers can improve medical diagnosis; however, a critical factor in nanosensor development is deciding which biomarker to target, as most diseases present several biomarkers. ...
-
Protocol
A Wearable Sensing System for Assessment of Exposures to Environmental Volatile Organic Compounds
A portable chemical sensing system that integrates sample preconcentration, separation and detection as well as wireless communication functionalities into a compact, wearable format can provide continuous and...
-
Protocol
Fluorescence Labeling of Short RNA by Oxidation at the 3′-End
In RNA nanotechnology, construction of nanoparticles involves conjugation of functionalities, cross-linking of modules, labeling of RNA subunits, and chemical modification of nucleotides. Efficiency and sensit...
-
Protocol
Vitrification of Heart Valve Tissues
Application of the original vitrification protocol used for pieces of heart valves to intact heart valves has evolved over time. Ice-free cryopreservation by Protocol 1 using VS55 is limited to small samples w...
