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Article
Inhibition of biphasic ethylene production enhances tolerance to abiotic stress by reducing the accumulation of reactive oxygen species in Nicotiana tabacum
Reactive oxygen species (ROS), such as H2O2, are important plant cell signaling molecules involved in responses to biotic and abiotic stresses and in developmental and physiological processes. Despite the well-kn...
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Article
Development of 3-D poly(trimethylenecarbonate-co-ε-caprolactone)-block-poly(p-dioxanone) scaffold for bone regeneration with high porosity using a wet electrospinning method
The three dimensional (3-D) poly(trimethylenecarbonate-co-ε-caprolactone)-block-poly(p-dioxanone) scaffold was made using a wet electrospinning method and its application as a tissue engineered matrix was evaluat...
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Article
1,3-Dichloro-2-propanol induces apoptosis via both calcium and ROS in mouse melanoma cells
We demonstrated that the apoptotic cell death of mouse melanoma cells exposed to 1,3-dichloro-2-propanol (DCP) was Ca2+ dependent. 1,3-DCP inhibited the growth of mouse melanoma cells in a concentration-dependent...
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Article
Quantitative and rapid analysis of transglutaminase activity using protein arrays in mammalian cells
We developed a novel on-chip activity assay using protein arrays for quantitative and rapid analysis of transglutami-nase activity in mammalian cells. Transglutaminases are a family of Ca2+-dependent enzymes invo...
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Article
Development of 3-D nanofibrous fibroin scaffold with high porosity by electrospinning: implications for bone regeneration
We made a three-dimensional (3-D) nanofibrous fibroin scaffold (NFS) with high porosity (94%) and examined its feasibility in bone regeneration. Under scanning electron microscopy, MC3T3-E1 preosteoblasts on t...
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Article
Characterization of terminal-repeat retrotransposon in miniature (TRIM) in Brassica relatives
We have newly identified five Terminal-repeat retrotransposon in miniature (TRIM) families, four from Brassica and one from Arabidopsis. A total of 146 elements, including three Arabidopsis families reported befo...
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Article
Intracellular drug delivery using poly(d,l-lactide-co-glycolide) nano- particles derivatized with a peptide from a transcriptional activator protein of HIV-1
Biodegradable poly(d,l-lactide-co-glycolide) (PLGA) nanoparticles were derivatized with Tat49−57 peptide, which is the protein transduction domain from the transcriptional activator Tat protein of human immunodef...
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Article
Removal of attenuator region of thr operon increases the production of threonine in Escherichia coli
Expression of the threonine (thr) operon of Escherichia coli is regulated by an attenuation mechanism. The regulatory portion of the thr operon contains a region coding for leader peptide that includes both conse...
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Article
Enhanced specific antibody productivity of hybridomas resulting from hyperosmotic stress is cell line-specific
Hybridomas with non-growth-associated antibody production are thought to exhibit enhanced specific monoclonal antibody productivity (q MAb) when subjected to hyperosmotic stress. Two hybridoma cell lines exhibiti...