407 Result(s)

Article

Expression and Purification of N and E Proteins from Severe Acute Respiratory Syndrome (SARS)-Associated Coronavirus: a Comparative Study

Histidine-tagged N (rNH) and E (rEH) proteins of Severe Acute Respiratory Syndrome (SARS)-coronovirus were expressed in the baculovirus/insect cell system and purified by immobilized metal affinity chromatogra...

Chia-Wei Lai, Yao-Chi Chung, Yiu-Kay Lai, Margaret Dah-Tsyr Chang… in Biotechnology Letters (2005)

Article

The effector caspases drICE and dcp-1 have partially overlap** functions in the apoptotic pathway in Drosophila

Caspases are essential components of the apoptotic machinery in both vertebrates and invertebrates. Here, we report the isolation of a mutant allele of the Drosophila effector caspase drICE as a strong suppressor...

D Xu, Y Wang, R Willecke, Z Chen, T Ding, A Bergmann in Cell Death & Differentiation (2006)

Article

ARK, the Apaf-1 related killer in Drosophila, requires diverse domains for its apoptotic activity

In mammals and Drosophila, apoptotic caspases are under positive control of the CED-4-like proteins Apaf-1 and ARK, respectively. In an EMS-mutagenesis screen, we isolated 33 ark mutants as recessive suppressors ...

M Srivastava, H Scherr, M Lackey, D Xu, Z Chen, J Lu… in Cell Death & Differentiation (2007)

Chapter and Conference Paper

Using Efficient RBF Network to Identify Interface Residues Based on PSSM Profiles and Biochemical Properties

Protein-protein interactions play a very important role in many biological processes, for example, information transfer along signaling pathways, and enzyme catalysis. Recently, scientists tried to predict the...

Yu-Yen Ou, Shu-An Chen, Chung-Lu Shao… in Pattern Recognition in Bioinformatics (2007)

Article

Eriocalyxin B induces apoptosis of t(8;21) leukemia cells through NF-κB and MAPK signaling pathways and triggers degradation of AML1-ETO oncoprotein in a caspase-3-dependent manner

Diterpenoids isolated from Labiatae family herbs have strong antitumor activities with low toxicity. In this study, Eriocalyxin B (EriB), a diterpenoid extracted from Isodon eriocalyx, was tested on human leukemi...

L Wang, W-L Zhao, J-S Yan, P Liu, H-P Sun, G-B Zhou… in Cell Death & Differentiation (2007)

Article

Binding of Sudan II and IV to lecithin liposomes and E. coli membranes: insights into the toxicity of hydrophobic azo dyes

Sudan red compounds are hydrophobic azo dyes, still used as food additives in some countries. However, they have been shown to be unsafe, causing tumors in the liver and urinary bladder in rats. They have been...

Lu Li, Hong-Wen Gao, Jiao-Rong Ren, Ling Chen, Yu-Cheng Li… in BMC Structural Biology (2007)

Article

A recombinant pseudorabies virus co-expressing capsid proteins precursor P1-2A of FMDV and VP2 protein of porcine parvovirus: a trivalent vaccine candidate

Pseudorabies (PR), foot-and-mouth disease (FMD), and porcine parvovirus disease are three important infectious diseases in swine worldwide. The gene-deleted pseudorabies virus (PRV) has been used as a live-vir...

Qi Hong, ** Qian, **ang-Min Li, **ao-Lan Yu, Huan-Chun Chen in Biotechnology Letters (2007)

Article

A rice phenomics study—phenotype scoring and seed propagation of a T-DNA insertion-induced rice mutant population

With the completion of the rice genome sequencing project, the next major challenge is the large-scale determination of gene function. As an important crop and a model organism, rice provides major insights in...

Chyr-Guan Chern, Ming-Jen Fan, Su-May Yu, Ai-Ling Hour… in Plant Molecular Biology (2007)

Article

Oxidative stress induces autophagic cell death independent of apoptosis in transformed and cancer cells

Autophagy is a self-digestion process that degrades intracellular structures in response to stresses leading to cell survival. When autophagy is prolonged, this could lead to cell death. Generation of reactive...

Y Chen, E McMillan-Ward, J Kong, S J Israels, S B Gibson in Cell Death & Differentiation (2008)

Article

Immobilization of Escherichia coli novablue γ-glutamyltranspeptidase in Ca-alginate-k-carrageenan beads

Chih-Peng Hung, Huei-Fen Lo, Wen-Hwei Hsu… in Applied Biochemistry and Biotechnology (2009)

Article

Interaction of perfluorooctanoic acid with human serum albumin

Recently, perfluorooctanoic acid (PFOA) has become a significant issue in many aspects of environmental ecology, toxicology, pathology and life sciences because it may have serious effects on the endocrine, im...

Ling-Ling Wu, Hong-Wen Gao, Nai-Yun Gao, Fang-Fang Chen… in BMC Structural Biology (2009)

Article

Baifuzi reduces transient ischemic brain damage through an interaction with the STREX domain of BK_Ca channels

Stroke is a long-term disability and one of the leading causes of death. However, no successful therapeutic intervention is available for the majority of stroke patients. In this study, we explored a tradition...

S Chi, W Cai, P Liu, Z Zhang, X Chen, L Gao, J Qi, L Bi, L Chen… in Cell Death & Disease (2010)

Article

Three-dimensional domain swap** as a mechanism to lock the active conformation in a super-active octamer of SARS-CoV main protease

Proteolytic processing of viral polyproteins is indispensible for the lifecycle of coronaviruses. The main protease (M^pro) of SARS-CoV is an attractive target for anti-SARS drug development as it is essential for...

Shengnan Zhang, Nan Zhong, Fei Xue, Xue Kang, **aobai Ren, Jiaxuan Chen… in Protein & Cell (2010)

Article

Bioinformatic analysis of the human DHRS4 gene cluster and a proposed mechanism for its transcriptional regulation

The human DHRS4 gene cluster consists of three genes, DHRS4, DHRS4L2 and DHRS4L1. Among them, DHRS4 encodes NADP(H)-dependent retinol dehydrogenase/reductase. In a previous study, we investigated the alternative ...

Zhong-**g Su, Qiao-**a Zhang, Ge-Fei Liu, Xu-Hong Song, Qi Li… in BMC Molecular Biology (2010)

Article

Close relationship between the 2009 H1N1 virus and South Dakota AIV strains

Although previous publications suggest the 2009 pandemic influenza A (H1N1) virus was reassorted from swine viruses of North America and Eurasia, the immediate ancestry still remains elusive due to the big evo...

Cun Li, ** An, Zhi-qiang Mi, Da-bin Liu, Huan-huan Jiang… in Virologica Sinica (2011)

Article

PIKA provides an adjuvant effect to induce strong mucosal and systemic humoral immunity against SARS-CoV

Severe Acute Respiratory Syndrome (SARS) is a deadly infectious disease caused by SARS Coronavirus (SARS-CoV). Inactivated SARS-CoV has been explored as a vaccine against SARS-CoV. However, safe and potent adj...

Wei-wei Gai, Yan Zhang, Di-han Zhou, Yao-qing Chen, **g-yi Yang… in Virologica Sinica (2011)

Article

DAPK activates MARK1/2 to regulate microtubule assembly, neuronal differentiation, and tau toxicity

Death-associated protein kinase (DAPK) is a key player in several modes of neuronal death/injury and has been implicated in the late-onset Alzheimer's disease (AD). DAPK promotes cell death partly through its ...

P-R Wu, P-I Tsai, G-C Chen, H-J Chou, Y-P Huang, Y-H Chen… in Cell Death & Differentiation (2011)

Article

Fork head transcription factor is required for ovarian mature in the brown planthopper, Nilaparvata lugens (Stål)

The brown planthopper (BPH), Nilaparvata lugens, is the most devastating rice pest in many areas throughout Asia. The reproductive system of female N. lugens consists of a pair of ovaries with 24-33 ovarioles per...

**aolin Dong, Yifan Zhai, Jianqing Zhang, Zhongxiang Sun… in BMC Molecular Biology (2011)

Article

A point mutation in the DNA-binding domain of HPV-2 E2 protein increases its DNA-binding capacity and reverses its transcriptional regulatory activity on the viral early promoter

The human papillomavirus (HPV) E2 protein is a multifunctional DNA-binding protein. The transcriptional activity of HPV E2 is mediated by binding to its specific binding sites in the upstream regulatory region...

Chen Gao, Ming-Ming Pan, Yan-Jun Lei, Li-Qing Tian, Hui-Ying Jiang… in BMC Molecular Biology (2012)

Article

Ablation of LMO4 in glutamatergic neurons impairs leptin control of fat metabolism

The LIM domain only 4 (LMO4) protein is expressed in the hypothalamus, but its function there is not known. Using mice with LMO4 ablated in postnatal glutamatergic neurons, including most neurons of the parave...

Xun Zhou, Mariana Gomez-Smith, Zhaohong Qin… in Cellular and Molecular Life Sciences (2012)