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Prediction of Human MicroRNA Targets

  • Protocol
MicroRNA Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 342))

Abstract

MicroRNAs (miRNAs) are small, nonprotein-coding RNAs that regulate gene expression. Although hundreds of human miRNA genes have been discovered, the functions of most of these are unknown. Computational predictions indicate that miRNAs, which account for at least 1% of human protein-coding genes, regulate protein production for thousands of or possibly all of human genes. We discuss the functions of mammalian miRNAs and the experimental and computational methods used to detect and predict human miRNA target genes. Anticipating their impact on genome-wide discovery of miRNA targets, we describe the various computational tools and web-based resources available to predict miRNA targets.

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Authors and Affiliations

  1. Computational Biology Center, Memorial Sloan-Kettering Cancer Center, New York, NY

    Bino John & Chris Sander

  2. Department of Systems Biology, Harvard Medical School, Boston, MA

    Debora S. Marks

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  1. Bino John
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  2. Chris Sander
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  3. Debora S. Marks
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Editors and Affiliations

  1. Department of Cell and Neurobiology, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA

    Shao-Yao Ying

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© 2006 Humana Press Inc.

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John, B., Sander, C., Marks, D.S. (2006). Prediction of Human MicroRNA Targets. In: Ying, SY. (eds) MicroRNA Protocols. Methods in Molecular Biology™, vol 342. Humana Press. https://doi.org/10.1385/1-59745-123-1:101

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  • DOI: https://doi.org/10.1385/1-59745-123-1:101

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-581-1

  • Online ISBN: 978-1-59745-123-9

  • eBook Packages: Springer Protocols

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