Abstract
The polymerase chain reaction (PCR) has rapidly become an essential tool within the diagnostic laboratory. Therefore, it is crucial when setting up a new PCR-based test to ensure that the PCR reaction is carefully designed to be as robust and reliable as possible. Usually, little optimization is required. However, there are some instances when a particular region of DNA proves difficult to amplify by PCR. A number of factors are important to consider when choosing PCR conditions, and these are discussed in this chapter.
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© 2004 Humana Press Inc.
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Bulman, M.P. (2004). Optimizing PCR for Clinical Diagnosis. In: Elles, R., Mountford, R. (eds) Molecular Diagnosis of Genetic Diseases. Methods in Molecular Medicine™, vol 92. Humana Press, Totowa, NJ. https://doi.org/10.1385/1-59259-432-8:1
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DOI: https://doi.org/10.1385/1-59259-432-8:1
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-0-89603-932-2
Online ISBN: 978-1-59259-432-0
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